Red light-triggered nucleic acid-templated reaction based on cyclic oligonucleotide substrates

A red light-triggered reaction based on cyclic oligonucleotide substrates that is accelerated over 30-fold by specific nucleic acid templates and generates a bright fluorescent probe was developed. We confirmed that this reaction is compatible with fluorescence correlation spectroscopy (FCS) thereby allowing detection of nucleic acids down to 1 nM. We developed a red light-triggered, fluorogenic chemical reaction based on cyclic oligonucleotide substrates that is accelerated over 30-fold by nucleic acid templates that allows quick and sequence specific detection of nucleic acid down to 1 nM.