Beacon-mediated exponential amplification reaction (BEAR) using a single enzyme and primer

Beacon-mediated exponential amplification reaction (BEAR) using a single enzyme and primer

Beacon-mediated Exponential Amplification Reaction ( BEAR ) enables isothermal, exponential signal amplification. BEAR uses only a single enzyme and a single primer. Detection of 0.2 amol of a mitochondrial DNA with a point mutation in less than an hour demonstrates an application of the BEAR techni...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2019-09, Vol.55 (72), p.1677-168
Hauptverfasser: Newbigging, Ashley M, Zhang, Hongquan, Le, X. Chris
Format: Artikel
Sprache:eng
Schlagworte:
Amplification
Enzymes
Mitochondrial DNA
Mutation
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Beschreibung
Zusammenfassung:Beacon-mediated Exponential Amplification Reaction ( BEAR ) enables isothermal, exponential signal amplification. BEAR uses only a single enzyme and a single primer. Detection of 0.2 amol of a mitochondrial DNA with a point mutation in less than an hour demonstrates an application of the BEAR technique for nucleic acid research. Beacon-mediated Exponential Amplification Reaction ( BEAR ) enables isothermal, exponential signal amplification. BEAR uses only a single enzyme and a single primer.
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc04226a