A New Hemicyanine-based Fluorophore for Monitoring pH and Lysosome Imaging

On account of long wavelength emission and high stability, a hemi-cyanine skeleton of 2,3,3-trimethyl-1-(3-sulfopropyl)-3H-benzo[f]indolinium was condensed with 6-hydroxy-7-morpholin-4-yl-methyl-naphthalene-2-carbaldehyde in the presence of acetic anhydride. The new probe displays a sensitive absorp...

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Veröffentlicht in:	Journal of analytical chemistry (New York, N.Y.) N.Y.), 2019-09, Vol.74 (9), p.940-944
Hauptverfasser:	Yingjie Lei, Wu, Xinshi, Yao, Qingjia
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Chemistry Chemistry and Materials Science Commercialization Correlation coefficients Emission Fluorescence Fluorescent indicators Hydrogen-ion concentration Ion migration Linearity Naphthalene
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Zusammenfassung:	On account of long wavelength emission and high stability, a hemi-cyanine skeleton of 2,3,3-trimethyl-1-(3-sulfopropyl)-3H-benzo[f]indolinium was condensed with 6-hydroxy-7-morpholin-4-yl-methyl-naphthalene-2-carbaldehyde in the presence of acetic anhydride. The new probe displays a sensitive absorption and fluorescence according to the pH value changes and exhibits an excellent linearity of the relative emission intensity ( I 670 / I 580 ) vs. pH in the pH range of 7.0–9.5 with a correlation coefficient of 0.993. Moreover, compared to the commercialized LysoTracker Green DND-26 the probe is targeting to the lysosomal imaging in HeLa cells and, thus, can be further used as a near-infrared fluorescent probe for cellular research.
ISSN:	1061-9348 1608-3199
DOI:	10.1134/S1061934819090119