Specific recognition of ribavirin in animal‐derived foods by high performance liquid chromatography combined with magnetic solid‐phase extraction based on highly selective Zr‐Fe3O4

For the first time, a magnetic solid‐phase extraction with high‐performance liquid chromatography detection method using Zr functionalized Fe3O4 magnetic material to enrich ribavirin was successfully established. Zr components that modified in Fe3O4 nanoparticles via a simple one‐step hydrothermal method was selected in this work to specifically capture ribavirin by the strong chemical bonding between Zr components of Zr functionalized Fe3O4 magnetic material and cis‐hydroxyl of ribavirin, which was confirmed by pseudo‐second‐order kinetic model. And Fe3O4 components were selected in this work to achieve simple operation. Under the optimal experimental conditions, proposed magnetic solid‐phase extraction with high‐performance liquid chromatography detection method along with Zr functionalized Fe3O4 magnetic material offered a wide range linearity at 10–200 µg/L with correlation coefficient of 0.9978 with low detection limit of 2.68 µg/L for ribavirin. The relative standard deviations obtained from nine parallel extractions of 100 µg/L ribavirin were 4.41% and revealed good repeatability. This established method was successfully applied to detect real samples including chicken liver, egg, and shrimp with satisfactory recoveries of 74.13–92.9%.