ALKALINE PROTEASE PURIFICATED FROM WILD TYPE BACILLUS SP: CHARACTERIZATION AND APPLICATION IN DETERGENT INDUSTRY

ALKALINE PROTEASE PURIFICATED FROM WILD TYPE BACILLUS SP: CHARACTERIZATION AND APPLICATION IN DETERGENT INDUSTRY

Bacillus subtilis, an alkaline protease producing strain isolated from soil sample, was identified by 16S rRNA gene sequencing method and its biochemical properties. The optimum pH and temperature of the enzyme were found as 9.5 and 60°C, respectively. The enzyme showed excellent stability with some...

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Veröffentlicht in:Fresenius environmental bulletin 2019-07, Vol.28 (7), p.5356
Hauptverfasser: Ozdenefe, Melis Sumengen, Dincer, Sadik, Unal, Mustafa Umit, Mercimek Takci, Hatice Aysun, Ozyapici, Afet Arkut, Kayis, Fikret Buyukkaya
Format: Artikel
Sprache:eng
Schlagworte:
Alkaline protease
Bacillus subtilis
Detergent industry
Detergents
Enzymes
Gene sequencing
Laundry
Protease
rRNA 16S
Stability analysis
Washing powders
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Zusammenfassung:Bacillus subtilis, an alkaline protease producing strain isolated from soil sample, was identified by 16S rRNA gene sequencing method and its biochemical properties. The optimum pH and temperature of the enzyme were found as 9.5 and 60°C, respectively. The enzyme showed excellent stability with some commercial liquid laundry detergents. Wash performance analysis revealed that B. subtilis crude protease could effectively remove bloodstains. Bacillus subtilis strain produced nine major extracellular proteases, which was revealed by zymography technique. Appraising its promising properties, Bacillus subtilis crude enzyme can be considered as a potential candidate for future use in detergent industries.
ISSN:1018-4619
1610-2304