Evaluation of In vitro Anti-Diabetic and Anti-Oxidant activities and Preliminary Phytochemical screening of Gel, Epidermis and Flower extract of Aloe vera

Diabetes mellitus is a metabolic disorder that may lead to overproduction of reactive oxygen species (ROS) in endothelial cells which contributes the pathogenesis of various complications1. Chemicals: Ascorbic acid, Gallic acid, quercetin, aluminium chloride, ferric chloride (FeCL); Folin Ciocalteu reagent; bovine serum albumin (BSA); potassium persulphate; 2,2-diphenyl-1-picrylhy-drazyl (DPPH), trichloroacetic acid (TCA) 2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS), Hydrogen peroxide (H2O2) were purchased from Sigma chemicals (St. Louis, MO, USA). [...]in the presence of gel, epidermis and flower extracts, plasmid DNA is protected from the damages taking place in the presence of UV and H2O2.The presence of different phenolic constituents present in all the three extracts which was analyzed by RP-HPLC may suppress the formation of linear plasmid DNA and protected damage. [...]the present comparative study of Aloe vera gel, epidermis and flower extracts showed significant antioxidant and scavenging activities of DPPH, ABTS, H2O2, metal chelating activity and protected the DNA damage.