Synthesis, Characterization of 2-Methylthio-1, 4-dihydropyrimidines for its Antibacterial potential

Label one rack set I- Control (blank), Gram positive Set IIStaphylococcus aureus, Set III- Bacillus Subtilis and gram negative Set IV-Escherichia coli, Set VProteus vulgaris. 2) Using a sterile 1 mL glass syringe, add 0.8 mL of DMSO in labeled 1 and 0.5 mL of DMSO labeled 2 through labeled 8 in Set I-V. 3) With a sterile 1 mL glass syringe, add 0.2 mL test compounds solution in labeled 1 test tube and mix it. 4) Using a sterile 1 mL glass syringe, transfer test compounds solution 0.5 mL from tube 1 to tube 2. C-2 modified dihydropyrimidine derivatives of type II was synthesized by alkylation reaction. [...]2-methylthio-1, 4-dihydropyrimidine derivatives were synthesized from the respective tetrahydropyrimidine-2-thiones by reaction with methyl iodide in the presence of pyridine (Scheme 1). The result suggests that the antibacterial activities are markedly influenced by the aromatic substituent. [...]compounds IIe, IIf, IIi, and IIj with electron withdrawing substituents in the aromatic ring, show greater antibacterial activity. [...]these compounds may be further exploited for other pharmacological activities.