Red Light Activates the [sigma]^sup B^-Mediated General Stress Response of Bacillus subtilis via the Energy Branch of the Upstream Signaling Cascade

The ...-dependent general stress response in the common soil bacterium Bacillus subtilis can be elicited by a range of stress factors, such as starvation or an ethanol, salt, or heat shock, via a complex upstream signaling cascade. Additionally, ... can be activated by blue light via the phototropin...

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Veröffentlicht in:Journal of bacteriology 2010-02, Vol.192 (3), p.755
Hauptverfasser: Ávila-Pérez, Marcela, van der Steen, Jeroen B, Kort, Remco, Hellingwerf, Klaas J
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Sprache:eng
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Zusammenfassung:The ...-dependent general stress response in the common soil bacterium Bacillus subtilis can be elicited by a range of stress factors, such as starvation or an ethanol, salt, or heat shock, via a complex upstream signaling cascade. Additionally, ... can be activated by blue light via the phototropin homologue YtvA, a component of the environmental branch of the signaling cascade. Here we use a reporter-gene fusion to show that ... can also be activated by red light via the energy branch of its upstream signaling cascade. Deletion mutagenesis and homologous overproduction experiments indicate that the RsbP protein (composed of an N-terminal Per-ARNT-Sim [PAS] domain and a C-terminal PP2C-type phosphatase domain) is involved in the red light response. This second light input pathway functions complementarily to YtvA; it shows broader spectral sensitivity but requires higher light intensities. These results are confirmed by transcriptome analyses, which show that both light effects result in upregulation of the ... regulon, with minimal activation of other responses. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0021-9193
1098-5530