Confirmatory Detection of Escherichia coli O157: H7 in Diarrhoeic and Non-Diarrhoeic Calves by using real time PCR with Studying the Antimicrobial Susceptibility of these bacteria

[...]the real time PCR can be used as a sensitive and reliable tool for diagnosis of E. coli O157:H7, and this bacteria showed high resistance to antibiotic. Bacterial Strain: Thirty two strains of E. coli O157:H7 were obtained from Dept. of Internal and Preventive Medicine/ College of Veterinary Medicine / University of Baghdad, which isolated from 350 faecal samples collected from non diarrheic and diarrheic calves which found in different places in Baghdad Province (Yousif and Hussein, 2015). [...]the Real time PCR assay described in this study is a sensitive and accurate method for specific detection and profiling of EHEC O157:H7 and this bacteria can be referred as Multi drug resistant bacteria due to its ability to resist most antimicrobial that used in this study. 6. [...]edition.; 2013: 246249.