Stability Indicating RP-HPLC-PDA Method for Simultameous Quantification of Glucosamine, Methyl Sulphonyl Methane and Diacerein Tablets

The chromatographic separation was performed on a reverse phase Altima C18 Column (150·4.6mm, 5pm particle size) consisting mobile phase of potassium dihydrogen ortho phosphate buffer: acetonitrile (65:35 v/v), with a flow rate lml/min, temperature 30°C and UV detection wavelength 254nm. [...]the authors developed a simple, fast, precise, accurate, robust and stability indicating method for simultaneous assay of Glucosamine, methyl sulphonyl methane and diacerein, in tablet dosage form. Robustness: Robustness of the developed analytical method refers to its ability to remain unaffected due to small but deliberate variations in the method parameters (flow rate, mobile phase ratio and column temperature) which indicates reliability of the method for routine analysis. The developed RP-HPLC-PDA method was found to be suitable for the analysis of glucosamine, methyl sulphonyl methane and diacerein in tablet dosage form and was found to be simple , reliable, economical and precise. [...]this RP-HPLC method for estimation of glucosamine, methyl sulphonyl methane and diacerein can be used in various laboratories for its quantitative determination in bulk and pharmaceutical tablet dosage form.