Construction of a high-density genetic linkage map and identification of quantitative trait loci associated with clubroot resistance in radish (Raphanus sativus L.)
Clubroot is a devastating disease caused by Plasmodiophora brassicae , leading to substantial yield loss of cruciferous plants, especially radish ( Raphanus sativus ) in China. Thus, there is a need to elucidate the mechanisms underlying the pathogenicity of P. brassicae and to breed clubroot-resist...
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Veröffentlicht in: | Molecular breeding 2019-08, Vol.39 (8), p.1-12, Article 116 |
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Zusammenfassung: | Clubroot is a devastating disease caused by
Plasmodiophora brassicae
, leading to substantial yield loss of cruciferous plants, especially radish (
Raphanus sativus
) in China. Thus, there is a need to elucidate the mechanisms underlying the pathogenicity of
P. brassicae
and to breed clubroot-resistant radish cultivars. In the present study, we constructed a high-density linkage map of
R. sativus
by restriction-site-associated DNA sequencing (RAD-Seq) using an F
2
population derived from a cross between the clubroot-resistant and clubroot-susceptible inbred lines “BJJ” and “XNQ,” respectively. The genetic map spans 794.3 cM and has 1148 SNPs distributed across nine linkage groups corresponding to the nine chromosomes of
R. sativus
. The average distance between the adjacent markers is 0.7 cM. A set of five QTLs (viz.,
RsCr1
,
RsCr2
,
RsCr3
,
RsCr4
, and
RsCr5
) associated with resistance to clubroot was detected on chromosomes 8 and 9. The limit of detection values of quantitative trait loci (QTLs) ranged from 5.23 to 7.65, accounting for 7.26–31.38% of the observed phenotypic variance. Synteny analysis showed that
RsCr1
is homologous to the clubroot resistance gene
Crr1
in
Brassica rapa
. This high-density genetic map of
R. sativus
provides valuable information for clubroot resistance gene selection and resistant variety breeding, while the QTL mapping results provide a reference dataset for effective gene exploration and marker-assisted breeding programs. |
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ISSN: | 1380-3743 1572-9788 |
DOI: | 10.1007/s11032-019-1020-5 |