ANTI-ACETYLCHOLINESTERASE, ANTIPROTOZOAL AND CYTOTOXIC ACTIVITIES OF SOME TURKISH MARINE ALGAE

The crude (MeOH:CHCl3) and n-hexane-soluble extracts from four brown algae (Phaeophyceae) [Petaloniafascia, Cystoseira crinita, Cystoseirafoeniculacea, and Halopteris scoparia], one red alga (Rhodophyta) [Jania rubens] and three green algae (Chlorophyta) [Chaetomorpha aerea, Codium fragile subsp. fragile, and Ulva compressa] from Turkish coasts (Izmir Bay, Ayvalik and Çanakkale) were assessed in vitro for their acetylcholinesterase (AChE) inhibitory activities at 200, 150, 50, 20 μg/mL test concentration with Ellman's method. The crude ex-tract of P. fascia possessed the highest inhibition (IC50 value of 19,22± 10,47 μg/mL) against AChE. Galanthamine HBr was used as standard drug that gave against AChE enzyme IC50 of 3.44±1.14 μM. In the second stage, the crude, hexane-soluble, chloroform-soluble and water-methanol soluble extracts of the marine algae were observed in vitro against parasitic protozoa (Plasmodium falciparum, Trypanosoma brucei brucei, Trypanosoma cruzi ve Leish-mania infantum). According to results, the most potent protozoal activities were shown by the Khex of C. crinita (IC50 value of 10,62 μg/ml), followed by the Khex of C. fragile subsp. fragile (IC50 value of 11,89 μg/ml). The hekzan-soluble (Khex) and chloroform-soluble (KCH) extracts gave the best results. The marine algae were also tested on MRC-5 cells (human fibroblasts) for by controlling tamoxifen. The extracts of H. scoparia, C. aerea ve C. fragile subsp. fragile showed toxicity.