De novo transcriptome analysis of Fraxinus velutina Torr in response to NaCl stress
This study aimed to further clarify the mechanisms of salt-alkali tolerance in Fraxinus velutina Torr. Shoots obtained from Fraxinus velutina Torr (also known as velvet ash or Arizona ash) and of Fraxinus chinensis Roxb (controls) were planted in a greenhouse with hydroponics and 1-year-old seedling...
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Veröffentlicht in: | Tree genetics & genomes 2019-08, Vol.15 (4), p.1-7, Article 56 |
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Sprache: | eng |
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Zusammenfassung: | This study aimed to further clarify the mechanisms of salt-alkali tolerance in
Fraxinus velutina
Torr. Shoots obtained from
Fraxinus velutina
Torr (also known as velvet ash or Arizona ash) and of
Fraxinus chinensis
Roxb (controls) were planted in a greenhouse with hydroponics and 1-year-old seedlings were irrigated with 40 mM NaCl, followed by 80 mM NaCl; young leaves were then harvested and sequenced from total RNA. Differential expression gene analyses of
Fraxinus velutina
Torr as well as that of
Fraxinus chinensis
Roxb before and after treatment were conducted. The Gene Ontology biological process (GO-BP) terms and pathways enriched by overlapping genes were analyzed, and the co-expression network of them was built. A total of 454 overlapping upregulated and 278 overlapping downregulated genes were found in the two comparisons. Upregulated genes were mainly enriched in GO-BP terms of alkaloid biosynthetic process, and the pathways such as Ras signaling pathway, while downregulated genes were primarily enriched in photosynthesis and phenylpropanoid biosynthesis. The processes of phenylpropanoid biosynthesis and photosynthesis, as well as metallothionein-like protein and photosystem I P700–related proteins, appear to be involved in salt stress tolerance in
Fraxinus velutina
Torr. |
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ISSN: | 1614-2942 1614-2950 |
DOI: | 10.1007/s11295-019-1340-y |