Expression and Stress Tolerance Analysis of NAC24 from Tamarix hispida

【Objective】 The NAC transcription factors are one of the largest families of plant-specific transcription factors, widely involved in plant growth and development process, and it plays a crucial role in the process responding to salt, drought and many other abiotic stresses. In present study, we cloned a NAC transcription factors gene from halophyte woody plant Tamarix hispida, to study its salt tolerance and drought resistance function in order to lay a theoretical foundation for studying the molecular mechanism of resistance to woody plant NAC transcription factors.【Method】 A NAC transcription factor gene was isolated from NaHCO3 stress transcriptome database of T. hispida, and it was named as ThNAC24（GenBank number: KF031949）. Bioinformatics tools were used to perform multiple sequence alignment and phylogenetic analysis of NAC proteins from nine other species and 105 NAC proteins in Arabidopsis thaliana were analyzed. The T. hispida samples were treated with 300 mmol·L-1 NaCl and 400 mmol·L-1 Mannitol res