Fluorescence Quenching of Alexa Fluor 488-labeled DNA by Complementary Trp-containing PNA Partitioned in Liquid-ordered Domains

Fluorescence Quenching of Alexa Fluor 488-labeled DNA by Complementary Trp-containing PNA Partitioned in Liquid-ordered Domains

We demonstrate Trp-containing PNA/Alexa Fluor 488 (AL488)-labeled DNA hybrid partitions preferentially into liquid-ordered (lo) microdomains of GUV. The Trp quenches the fluorescence of AL488 microscopically. The mechanism involved collisional and static quenching, both of which proceeded effectivel...

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Veröffentlicht in:Chemistry letters 2017-11, Vol.46 (11), p.1672-1675
Hauptverfasser: Oka, Yoshimi, Shishino, Hisae
Format: Artikel
Sprache:eng
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Fluorescence
Quenching
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Zusammenfassung:We demonstrate Trp-containing PNA/Alexa Fluor 488 (AL488)-labeled DNA hybrid partitions preferentially into liquid-ordered (lo) microdomains of GUV. The Trp quenches the fluorescence of AL488 microscopically. The mechanism involved collisional and static quenching, both of which proceeded effectively. The static quenching indicates an AL488–Trp complex through a stacking interaction.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.170755