A Photochemical Hydrogen Evolution System Combining Cyanobacterial Photosystem I and Platinum Nanoparticle-terminated Molecular Wires

A H2 generation system based on the cyanobacterial photosystem I (PSI) reconstituted with 1,4-naphthoquinone-stabilized Pt nanoparticles (PtNPs) was constructed. Irradiating the system with red light (680 nm) and using sodium l(+)-ascorbate as the sacrificial electron donor generated H2, which was detected by GC. The light-induced H2 generation was observed at a maximum rate of 0.026 mol H2 h−1 (mol PSI)−1 using 2,6-dichloroindophenol (DCIP) as the mediator. The H2 evolution was increased to 0.053 mol H2 h−1 (mol PSI)−1 in the presence of cytochrome c (cyt c) as the mediator.