The CUPRAC-paper microzone plates as a simple and rapid method for total antioxidant capacity determination of plant extract

The current work was aimed to develop the paper microzone plates (PµZP) as a scanometric system for determining the total antioxidant capacity of plant extracts. The PµZP was constructed by immobilizing CUPRAC reagent onto 70-well of patterned paper as a sensing zone for the scanometric detection system. The light blue-sensing zone showed a sensitive response to standard antioxidant (rutin) by forming light yellow color adducts which can be scanned and quantitatively measured by image processing program as a scanometric method. The sensor gave a notable signal at 8 min after rutin addition and presented a linear response in the concentration span of 1–10 mM. The developed method was shown to be reproducible (RSD