FuGENE® 6 Transfection Reagent: minimizing reagent-dependent side effects as analyzed by gene-expression profiling and cytotoxicity assays

Transfection protocols and the reagents used to deliver nucleic acids into cells can affect cell physiology, leading to misinterpretation of results. Using microarray analysis and cell viability assays, we evaluated different transfection reagents to determine their effects on gene expression profiles. Results show that FuGENE 6 Transfection Reagent exhibits very low cytotoxicity, delivers high transfection efficiency and generates few nonspecific side effects with minimal alteration in gene expression. [PUBLICATION ABSTRACT]