Random-centroid Optimization Reveals the Strongest Superoxide Anion Radical Scavenging Activity of Maltose- and Ribose-conjugated Chicken Myofibrillar Protein

Random-centroid optimization (RCO) was applied to determine the optimum preparative conditions for glycated chicken myofibrillar proteins (Mfs) conjugated to maltose or ribose. The optimization targets were antioxidative ability against superoxide anion radicals ( ) and a minimum 60% solubility in low ionic strength medium. Four optimization parameters, temperature, relative humidity (RH), reaction time, and quantity of maltose or ribose, were selected and 13 experimental conditions were obtained (using the RCO program). The examinations were independently assessed according to individual vertex values. The obtained optimal conditions for maltose and ribose were determined to be 61 and 38°C, 38 and 39% RH, 33.9 and 3.80 h reaction time, and a sugar to protein mixing ratio (w/w) of 5.59 and 10.4 (w/w), respectively, and scavenging activities of each conjugate reached the maximum of 274 ± 86 (n=3) and 368 ± 120 (n=3) units of superoxide dismutase/g of protein.