Purification and analysis of DNA from low DNA content food samples

Recently, molecular biology techniques are widely used in food analysis; yet, the complexity of food matrices causes difficulty in purification and analysis of the analyte. The aim of this study was to introduce a new material, zeolite Y, that is able to isolate DNA even from low DNA content food sa...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal für Verbraucherschutz und Lebensmittelsicherheit 2019-06, Vol.14 (2), p.147-155
Hauptverfasser: Altuntop Yayla, M. Esra, Ekinci Doğan, Canan, Sarioğlan, Şerife
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Recently, molecular biology techniques are widely used in food analysis; yet, the complexity of food matrices causes difficulty in purification and analysis of the analyte. The aim of this study was to introduce a new material, zeolite Y, that is able to isolate DNA even from low DNA content food samples; and, to demonstrate the integrity of isolated DNA via real-time polymerase chain reaction. Although the purity of the isolated DNA has to be improved with further work, Zeolite Y was shown to have a potential to be effectively used in DNA isolation for food analysis especially for low DNA content food samples.
ISSN:1661-5751
1661-5867
DOI:10.1007/s00003-018-1192-7