Purification and analysis of DNA from low DNA content food samples
Recently, molecular biology techniques are widely used in food analysis; yet, the complexity of food matrices causes difficulty in purification and analysis of the analyte. The aim of this study was to introduce a new material, zeolite Y, that is able to isolate DNA even from low DNA content food sa...
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Veröffentlicht in: | Journal für Verbraucherschutz und Lebensmittelsicherheit 2019-06, Vol.14 (2), p.147-155 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
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Online-Zugang: | Volltext |
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Zusammenfassung: | Recently, molecular biology techniques are widely used in food analysis; yet, the complexity of food matrices causes difficulty in purification and analysis of the analyte. The aim of this study was to introduce a new material, zeolite Y, that is able to isolate DNA even from low DNA content food samples; and, to demonstrate the integrity of isolated DNA via real-time polymerase chain reaction. Although the purity of the isolated DNA has to be improved with further work, Zeolite Y was shown to have a potential to be effectively used in DNA isolation for food analysis especially for low DNA content food samples. |
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ISSN: | 1661-5751 1661-5867 |
DOI: | 10.1007/s00003-018-1192-7 |