Development of Strain Discriminative Amplified Fragment Length Polymorpfic DNA for Bifidobacteria. Design of Strain-Specific Markers
Strain-discriminative differentiation of Bifidobacteria strains by help of Amplified Fragment Length Polymorphism (AFLP) with satisfactory discriminative power and reproducibility was developed. AFLP genotyping is based on restriction cleavage of DNA with enzyme couple Xho I and Taq I, specially des...
Gespeichert in:
| Veröffentlicht in: | Biotechnology, biotechnological equipment biotechnological equipment, 2012-01, Vol.26 (sup1), p.35-38 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 38 |
|---|---|
| container_issue | sup1 |
| container_start_page | 35 |
| container_title | Biotechnology, biotechnological equipment |
| container_volume | 26 |
| creator | Dimitrov, Zhechko |
| description | Strain-discriminative differentiation of Bifidobacteria strains by help of Amplified Fragment Length Polymorphism (AFLP) with satisfactory discriminative power and reproducibility was developed. AFLP genotyping is based on restriction cleavage of DNA with enzyme couple Xho I and Taq I, specially designed adapters, preselective and selective PCR primers. AFLP derived fragments were used successfully as a source of strain-specific markers for one probiotic Bifidobacterium longum strain, and subsequently, based on their specific sequences-for design of strain-specific probe. It's specificity was confirmed upon 70 Bifidobacteria strains. |
| doi_str_mv | 10.5504/50YRTIMB.2011.0007 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2229650303</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1017973642</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-3be5e2d7640845d7b85e92a00f3ce68f211706a9decec6199e3858ddd01111953</originalsourceid><addsrcrecordid>eNp9kT9vFDEQxVeISISEL0BlKQ3NHmN7vX-KFJccgUiXgEgoqCyfPb447NqLvRd0fT44vhwREgXVTPF7TzPvFcVbCjMhoHov4PvX28ursxkDSmcA0LwoDimnVckFh5dPO5Sspe2r4nVK9xkAoM1h8bjAB-zDOKCfSLDkZorKebJwSUc3OK8m94BkPoy9sw4NuYhq_cQu0a-nO_Il9NshxNE6TRbXc2JDJGcZNWGl9ITRqRlZYHJr_9e9vBlRu53iSsUfGNNxcWBVn_DNn3lUfLv4cHv-qVx-_nh5Pl-WuoJ6KvkKBTLT1BW0lTDNqhXYMQVguca6tYzSBmrVGdSoa9p1yFvRGmNyJpR2gh8V7_a-Yww_N5gmOeQ_se-Vx7BJkuZIuobXFcvoyT_ofdhEn6-TjLGuFsCBZ4rtKR1DShGtHHNqKm6zldwVI5-Lkbti5K6YLDrdi5zPaQ3qV4i9kZPa9iHaqLx2SfL_6H8DDxGVrA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2229650303</pqid></control><display><type>article</type><title>Development of Strain Discriminative Amplified Fragment Length Polymorpfic DNA for Bifidobacteria. Design of Strain-Specific Markers</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Dimitrov, Zhechko</creator><creatorcontrib>Dimitrov, Zhechko</creatorcontrib><description>Strain-discriminative differentiation of Bifidobacteria strains by help of Amplified Fragment Length Polymorphism (AFLP) with satisfactory discriminative power and reproducibility was developed. AFLP genotyping is based on restriction cleavage of DNA with enzyme couple Xho I and Taq I, specially designed adapters, preselective and selective PCR primers. AFLP derived fragments were used successfully as a source of strain-specific markers for one probiotic Bifidobacterium longum strain, and subsequently, based on their specific sequences-for design of strain-specific probe. It's specificity was confirmed upon 70 Bifidobacteria strains.</description><identifier>ISSN: 1310-2818</identifier><identifier>EISSN: 1314-3530</identifier><identifier>DOI: 10.5504/50YRTIMB.2011.0007</identifier><language>eng</language><publisher>Sofia: Taylor & Francis</publisher><subject>Adapters ; AFLP ; Amplification ; Amplified fragment length polymorphism ; Bifidobacteria ; Bifidobacterium longum ; Biomarkers ; Deoxyribonucleic acid ; DNA ; Genotyping ; Polymorphism ; Probiotics ; strain-specific markers ; Strains (organisms) ; Yogurt</subject><ispartof>Biotechnology, biotechnological equipment, 2012-01, Vol.26 (sup1), p.35-38</ispartof><rights>2012 Taylor and Francis Group, LLC 2012</rights><rights>2012 Taylor and Francis Group, LLC</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-3be5e2d7640845d7b85e92a00f3ce68f211706a9decec6199e3858ddd01111953</citedby><cites>FETCH-LOGICAL-c406t-3be5e2d7640845d7b85e92a00f3ce68f211706a9decec6199e3858ddd01111953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Dimitrov, Zhechko</creatorcontrib><title>Development of Strain Discriminative Amplified Fragment Length Polymorpfic DNA for Bifidobacteria. Design of Strain-Specific Markers</title><title>Biotechnology, biotechnological equipment</title><description>Strain-discriminative differentiation of Bifidobacteria strains by help of Amplified Fragment Length Polymorphism (AFLP) with satisfactory discriminative power and reproducibility was developed. AFLP genotyping is based on restriction cleavage of DNA with enzyme couple Xho I and Taq I, specially designed adapters, preselective and selective PCR primers. AFLP derived fragments were used successfully as a source of strain-specific markers for one probiotic Bifidobacterium longum strain, and subsequently, based on their specific sequences-for design of strain-specific probe. It's specificity was confirmed upon 70 Bifidobacteria strains.</description><subject>Adapters</subject><subject>AFLP</subject><subject>Amplification</subject><subject>Amplified fragment length polymorphism</subject><subject>Bifidobacteria</subject><subject>Bifidobacterium longum</subject><subject>Biomarkers</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Genotyping</subject><subject>Polymorphism</subject><subject>Probiotics</subject><subject>strain-specific markers</subject><subject>Strains (organisms)</subject><subject>Yogurt</subject><issn>1310-2818</issn><issn>1314-3530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kT9vFDEQxVeISISEL0BlKQ3NHmN7vX-KFJccgUiXgEgoqCyfPb447NqLvRd0fT44vhwREgXVTPF7TzPvFcVbCjMhoHov4PvX28ursxkDSmcA0LwoDimnVckFh5dPO5Sspe2r4nVK9xkAoM1h8bjAB-zDOKCfSLDkZorKebJwSUc3OK8m94BkPoy9sw4NuYhq_cQu0a-nO_Il9NshxNE6TRbXc2JDJGcZNWGl9ITRqRlZYHJr_9e9vBlRu53iSsUfGNNxcWBVn_DNn3lUfLv4cHv-qVx-_nh5Pl-WuoJ6KvkKBTLT1BW0lTDNqhXYMQVguca6tYzSBmrVGdSoa9p1yFvRGmNyJpR2gh8V7_a-Yww_N5gmOeQ_se-Vx7BJkuZIuobXFcvoyT_ofdhEn6-TjLGuFsCBZ4rtKR1DShGtHHNqKm6zldwVI5-Lkbti5K6YLDrdi5zPaQ3qV4i9kZPa9iHaqLx2SfL_6H8DDxGVrA</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Dimitrov, Zhechko</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7ST</scope><scope>7XB</scope><scope>8FD</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>SOI</scope><scope>7QL</scope><scope>7TM</scope></search><sort><creationdate>20120101</creationdate><title>Development of Strain Discriminative Amplified Fragment Length Polymorpfic DNA for Bifidobacteria. Design of Strain-Specific Markers</title><author>Dimitrov, Zhechko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-3be5e2d7640845d7b85e92a00f3ce68f211706a9decec6199e3858ddd01111953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adapters</topic><topic>AFLP</topic><topic>Amplification</topic><topic>Amplified fragment length polymorphism</topic><topic>Bifidobacteria</topic><topic>Bifidobacterium longum</topic><topic>Biomarkers</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Genotyping</topic><topic>Polymorphism</topic><topic>Probiotics</topic><topic>strain-specific markers</topic><topic>Strains (organisms)</topic><topic>Yogurt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dimitrov, Zhechko</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Environment Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Biotechnology, biotechnological equipment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dimitrov, Zhechko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of Strain Discriminative Amplified Fragment Length Polymorpfic DNA for Bifidobacteria. Design of Strain-Specific Markers</atitle><jtitle>Biotechnology, biotechnological equipment</jtitle><date>2012-01-01</date><risdate>2012</risdate><volume>26</volume><issue>sup1</issue><spage>35</spage><epage>38</epage><pages>35-38</pages><issn>1310-2818</issn><eissn>1314-3530</eissn><abstract>Strain-discriminative differentiation of Bifidobacteria strains by help of Amplified Fragment Length Polymorphism (AFLP) with satisfactory discriminative power and reproducibility was developed. AFLP genotyping is based on restriction cleavage of DNA with enzyme couple Xho I and Taq I, specially designed adapters, preselective and selective PCR primers. AFLP derived fragments were used successfully as a source of strain-specific markers for one probiotic Bifidobacterium longum strain, and subsequently, based on their specific sequences-for design of strain-specific probe. It's specificity was confirmed upon 70 Bifidobacteria strains.</abstract><cop>Sofia</cop><pub>Taylor & Francis</pub><doi>10.5504/50YRTIMB.2011.0007</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1310-2818 |
| ispartof | Biotechnology, biotechnological equipment, 2012-01, Vol.26 (sup1), p.35-38 |
| issn | 1310-2818 1314-3530 |
| language | eng |
| recordid | cdi_proquest_journals_2229650303 |
| source | EZB-FREE-00999 freely available EZB journals |
| subjects | Adapters AFLP Amplification Amplified fragment length polymorphism Bifidobacteria Bifidobacterium longum Biomarkers Deoxyribonucleic acid DNA Genotyping Polymorphism Probiotics strain-specific markers Strains (organisms) Yogurt |
| title | Development of Strain Discriminative Amplified Fragment Length Polymorpfic DNA for Bifidobacteria. Design of Strain-Specific Markers |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T18%3A57%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20Strain%20Discriminative%20Amplified%20Fragment%20Length%20Polymorpfic%20DNA%20for%20Bifidobacteria.%20Design%20of%20Strain-Specific%20Markers&rft.jtitle=Biotechnology,%20biotechnological%20equipment&rft.au=Dimitrov,%20Zhechko&rft.date=2012-01-01&rft.volume=26&rft.issue=sup1&rft.spage=35&rft.epage=38&rft.pages=35-38&rft.issn=1310-2818&rft.eissn=1314-3530&rft_id=info:doi/10.5504/50YRTIMB.2011.0007&rft_dat=%3Cproquest_cross%3E1017973642%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2229650303&rft_id=info:pmid/&rfr_iscdi=true |