Development of Strain Discriminative Amplified Fragment Length Polymorpfic DNA for Bifidobacteria. Design of Strain-Specific Markers

Strain-discriminative differentiation of Bifidobacteria strains by help of Amplified Fragment Length Polymorphism (AFLP) with satisfactory discriminative power and reproducibility was developed. AFLP genotyping is based on restriction cleavage of DNA with enzyme couple Xho I and Taq I, specially designed adapters, preselective and selective PCR primers. AFLP derived fragments were used successfully as a source of strain-specific markers for one probiotic Bifidobacterium longum strain, and subsequently, based on their specific sequences-for design of strain-specific probe. It's specificity was confirmed upon 70 Bifidobacteria strains.