COMPARATIVE BIOINFORMATICS ANALYSIS OF SOME VITIS SPECIES STILBENE SYNTHASE PROTEINS

In this study, bioinformatics analysis of stilbene synthase proteins found in some Vitis species were carried out employing bioinformatics tools. In the present study, stilbene synthase protein sequences belonging to some Vitis species were retrieved from NCBI in FASTA format. In bioinformatics analyses, amino acid number, molecular weight, instability index and GRAVY values were calculated with the Expasy!Protparam program. Phylogenetic analyses were conducted and genetic distance matrix was obtained via :tvfEGA 6.0 program using protein sequences. The 3D structure of stilbene synthase proteins was achieved by means of the PyMOL program. At the end of the study, it was found that the amino acid number of stilbene synthase proteins ranged between 392 and 399, molecular weight ranged between 4271 6.26 and 43791.55 Da, instability index ranged between 35.02 and 42.36 and GRAVY values ranged between -0.073 and -0.022. While Leu (10.46%) was the amino acid maximally found in stilbene synthase proteins on average, the amino acid at the minimum rate was found to be Trp (1.34%). In phylogenetic analyses performed using protein sequences, neighbours joining (NJ) tree consisted of 2 large clads. In the distance matrix, the lowest distance was found between Vitis vinifera and Vitis rotundifolia with 0.013, and the highest distance was found between Vitis pseudoreticulata and Vitis labrusca with 0.096. As a result, bioinformatics, phylogenetic and 3D protein analyses of some plant species carried out using stilbene synthase proteins will guide future bioinformatics and in silico studIes.