Preparation of temperature-sensitive magnetic microspheres for separation and purification of bromelain
•The core–shell temperature-responsive magnetic microspheres was designed for the first time.•The magnetic microspheres had the capability of thermal protection to the bromelain.•The magnetic microspheres showed excellent performance for purification of bromelain. The core–shell temperature-responsi...
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| Veröffentlicht in: | European management journal 2019-03, Vol.114 (2), p.253-262 |
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| container_title | European management journal |
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| creator | Han, Juan Wang, Li Wang, Yun Cai, Yunfeng Mao, Yanli Ni, Liang Xie, Xueqiao |
| description | •The core–shell temperature-responsive magnetic microspheres was designed for the first time.•The magnetic microspheres had the capability of thermal protection to the bromelain.•The magnetic microspheres showed excellent performance for purification of bromelain.
The core–shell temperature-responsive Fe3O4@P(GMA-co-NIPAM)/IDA/Ni2+ magnetic microspheres were prepared by distillation precipitation polymerization. The morphology, structure, and magnetic property of the magnetic microspheres were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), X-ray diffraction (XRD) and vibrating sample magnetometry (VSM). The obtained microspheres show remarkable affinity to bromelain with the maximum adsorption capacity was 117mg/g. The magnetic microspheres had the capability of thermal protection to the bromelain. UV–vis, FT-IR and circular dichroism (CD) spectra were determined to demonstrate that the process of adsorption and elution had no negative effect on the structure of bromelain. Finally, the microspheres were applied to purification of bromelain from the crude extract of pineapple peel with satisfactory results. |
| doi_str_mv | 10.1016/j.fbp.2019.01.008 |
| format | Article |
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The core–shell temperature-responsive Fe3O4@P(GMA-co-NIPAM)/IDA/Ni2+ magnetic microspheres were prepared by distillation precipitation polymerization. The morphology, structure, and magnetic property of the magnetic microspheres were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), X-ray diffraction (XRD) and vibrating sample magnetometry (VSM). The obtained microspheres show remarkable affinity to bromelain with the maximum adsorption capacity was 117mg/g. The magnetic microspheres had the capability of thermal protection to the bromelain. UV–vis, FT-IR and circular dichroism (CD) spectra were determined to demonstrate that the process of adsorption and elution had no negative effect on the structure of bromelain. Finally, the microspheres were applied to purification of bromelain from the crude extract of pineapple peel with satisfactory results.</description><identifier>ISSN: 0960-3085</identifier><identifier>ISSN: 0263-2373</identifier><identifier>EISSN: 1744-3571</identifier><identifier>EISSN: 1873-5681</identifier><identifier>DOI: 10.1016/j.fbp.2019.01.008</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Affinity ; Bromelain ; Core-shell structure ; Distillation precipitation polymerization ; Enzymes ; Extraction processes ; Fourier transforms ; Fruits ; Magnetic fields ; Magnetic microspheres ; Morphology ; Polymerization ; Property ; Purification ; Separation and purification ; Spectrum analysis ; Thermal protection</subject><ispartof>European management journal, 2019-03, Vol.114 (2), p.253-262</ispartof><rights>2019 Institution of Chemical Engineers</rights><rights>Copyright Elsevier Science Ltd. Apr 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c325t-84d85b2f5bd7633a435faf9ba932a4fccba9fb06dd4ddae9af49d5f07b10a56c3</citedby><cites>FETCH-LOGICAL-c325t-84d85b2f5bd7633a435faf9ba932a4fccba9fb06dd4ddae9af49d5f07b10a56c3</cites><orcidid>0000-0001-9472-0647</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960308518302918$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Han, Juan</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Wang, Yun</creatorcontrib><creatorcontrib>Cai, Yunfeng</creatorcontrib><creatorcontrib>Mao, Yanli</creatorcontrib><creatorcontrib>Ni, Liang</creatorcontrib><creatorcontrib>Xie, Xueqiao</creatorcontrib><title>Preparation of temperature-sensitive magnetic microspheres for separation and purification of bromelain</title><title>European management journal</title><description>•The core–shell temperature-responsive magnetic microspheres was designed for the first time.•The magnetic microspheres had the capability of thermal protection to the bromelain.•The magnetic microspheres showed excellent performance for purification of bromelain.
The core–shell temperature-responsive Fe3O4@P(GMA-co-NIPAM)/IDA/Ni2+ magnetic microspheres were prepared by distillation precipitation polymerization. The morphology, structure, and magnetic property of the magnetic microspheres were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), X-ray diffraction (XRD) and vibrating sample magnetometry (VSM). The obtained microspheres show remarkable affinity to bromelain with the maximum adsorption capacity was 117mg/g. The magnetic microspheres had the capability of thermal protection to the bromelain. UV–vis, FT-IR and circular dichroism (CD) spectra were determined to demonstrate that the process of adsorption and elution had no negative effect on the structure of bromelain. Finally, the microspheres were applied to purification of bromelain from the crude extract of pineapple peel with satisfactory results.</description><subject>Affinity</subject><subject>Bromelain</subject><subject>Core-shell structure</subject><subject>Distillation precipitation polymerization</subject><subject>Enzymes</subject><subject>Extraction processes</subject><subject>Fourier transforms</subject><subject>Fruits</subject><subject>Magnetic fields</subject><subject>Magnetic microspheres</subject><subject>Morphology</subject><subject>Polymerization</subject><subject>Property</subject><subject>Purification</subject><subject>Separation and purification</subject><subject>Spectrum analysis</subject><subject>Thermal protection</subject><issn>0960-3085</issn><issn>0263-2373</issn><issn>1744-3571</issn><issn>1873-5681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LxDAQxYMouK5-AG8Fz62TpukfPMmiq7CgBz2HNJmsKdumJu2C394sK3rzNDPw3puZHyHXFDIKtLztMtOOWQ60yYBmAPUJWdCqKFLGK3pKFtCUkDKo-Tm5CKEDAFpTviDbV4-j9HKybkicSSbsR4zj7DENOAQ72T0mvdwOOFmV9FZ5F8YP9BgS43wS_txy0Mk4e2us-o1rvetxJ-1wSc6M3AW8-qlL8v748LZ6Sjcv6-fV_SZVLOdTWhe65m1ueKurkjFZMG6kaVrZsFwWRqnYmRZKrQutJTbSFI3mBqqWguSlYktyc8wdvfucMUyic7Mf4kqR55TRvCwZjyp6VB2-CR6NGL3tpf8SFMSBp-hE5CkOPAVQEXlGz93Rg_H8vUUvgrI4KNTWo5qEdvYf9zfGJ4FJ</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Han, Juan</creator><creator>Wang, Li</creator><creator>Wang, Yun</creator><creator>Cai, Yunfeng</creator><creator>Mao, Yanli</creator><creator>Ni, Liang</creator><creator>Xie, Xueqiao</creator><general>Elsevier B.V</general><general>Elsevier Science Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8BJ</scope><scope>FQK</scope><scope>JBE</scope><orcidid>https://orcid.org/0000-0001-9472-0647</orcidid></search><sort><creationdate>20190301</creationdate><title>Preparation of temperature-sensitive magnetic microspheres for separation and purification of bromelain</title><author>Han, Juan ; Wang, Li ; Wang, Yun ; Cai, Yunfeng ; Mao, Yanli ; Ni, Liang ; Xie, Xueqiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-84d85b2f5bd7633a435faf9ba932a4fccba9fb06dd4ddae9af49d5f07b10a56c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Affinity</topic><topic>Bromelain</topic><topic>Core-shell structure</topic><topic>Distillation precipitation polymerization</topic><topic>Enzymes</topic><topic>Extraction processes</topic><topic>Fourier transforms</topic><topic>Fruits</topic><topic>Magnetic fields</topic><topic>Magnetic microspheres</topic><topic>Morphology</topic><topic>Polymerization</topic><topic>Property</topic><topic>Purification</topic><topic>Separation and purification</topic><topic>Spectrum analysis</topic><topic>Thermal protection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Juan</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Wang, Yun</creatorcontrib><creatorcontrib>Cai, Yunfeng</creatorcontrib><creatorcontrib>Mao, Yanli</creatorcontrib><creatorcontrib>Ni, Liang</creatorcontrib><creatorcontrib>Xie, Xueqiao</creatorcontrib><collection>CrossRef</collection><collection>International Bibliography of the Social Sciences (IBSS)</collection><collection>International Bibliography of the Social Sciences</collection><collection>International Bibliography of the Social Sciences</collection><jtitle>European management journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Juan</au><au>Wang, Li</au><au>Wang, Yun</au><au>Cai, Yunfeng</au><au>Mao, Yanli</au><au>Ni, Liang</au><au>Xie, Xueqiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of temperature-sensitive magnetic microspheres for separation and purification of bromelain</atitle><jtitle>European management journal</jtitle><date>2019-03-01</date><risdate>2019</risdate><volume>114</volume><issue>2</issue><spage>253</spage><epage>262</epage><pages>253-262</pages><issn>0960-3085</issn><issn>0263-2373</issn><eissn>1744-3571</eissn><eissn>1873-5681</eissn><abstract>•The core–shell temperature-responsive magnetic microspheres was designed for the first time.•The magnetic microspheres had the capability of thermal protection to the bromelain.•The magnetic microspheres showed excellent performance for purification of bromelain.
The core–shell temperature-responsive Fe3O4@P(GMA-co-NIPAM)/IDA/Ni2+ magnetic microspheres were prepared by distillation precipitation polymerization. The morphology, structure, and magnetic property of the magnetic microspheres were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), X-ray diffraction (XRD) and vibrating sample magnetometry (VSM). The obtained microspheres show remarkable affinity to bromelain with the maximum adsorption capacity was 117mg/g. The magnetic microspheres had the capability of thermal protection to the bromelain. UV–vis, FT-IR and circular dichroism (CD) spectra were determined to demonstrate that the process of adsorption and elution had no negative effect on the structure of bromelain. Finally, the microspheres were applied to purification of bromelain from the crude extract of pineapple peel with satisfactory results.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><doi>10.1016/j.fbp.2019.01.008</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-9472-0647</orcidid></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Affinity Bromelain Core-shell structure Distillation precipitation polymerization Enzymes Extraction processes Fourier transforms Fruits Magnetic fields Magnetic microspheres Morphology Polymerization Property Purification Separation and purification Spectrum analysis Thermal protection |
| title | Preparation of temperature-sensitive magnetic microspheres for separation and purification of bromelain |
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