Molecular chaperone prefoldin-assisted biosynthesis of gold nanoparticles with improved size distribution and dispersion

Here we report a novel aspect of molecular chaperone prefoldin (PFD) as a biomaterial in the biocatalytic synthesis of gold nanoparticles (AuNPs) using glycerol dehydrogenase (GLD). We found that PFD could inhibit the aggregation of AuNPs during the biosynthesis, leading to the formation of AuNPs with controlled size distribution. We demonstrated molecular chaperone prefoldin-assisted biocatalytic synthesis of size-controlled and dispersed gold nanoparticles using glycerol dehydrogenase enzyme.