Interaction of nano-TiO₂ with lysozyme: insights into the enzyme toxicity of nanosized particles
Background, aim, and scope Nanomaterials have been used increasingly in industrial production and daily life, but their human exposure may cause health risks. The interactions of nanomaterial with functional biomolecules are often applied as a precondition for its cytotoxicity and organ toxicity whe...
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| description | Background, aim, and scope Nanomaterials have been used increasingly in industrial production and daily life, but their human exposure may cause health risks. The interactions of nanomaterial with functional biomolecules are often applied as a precondition for its cytotoxicity and organ toxicity where various proteins have been investigated in the past years. In the present study, nano-TiO₂ was selected as the representative of nanomaterials and lysozyme as a representative for enzymes. By investigating their interaction by various instrumentations, the objective is to identify the action sites and types, estimate the effect on the enzyme structure and activity, and reveal the toxicity mechanism of nanomaterial. Materials and methods Laboratory-scale experiments were carried out to investigate the interactions of nano-TiO₂ with lysozyme. The interaction of nano-TiO₂ particles with lysozyme has been studied in the analogous physiological media in detail by UV spectrometry, fluorophotometry, circular dichroism (CD), scanning electron microscope, ζ-potential, and laser particle size. Results The interaction accorded with the Langmuir isothermal adsorption and the saturation number of lysozyme is determined to be 580 per nano-TiO₂ particle (60 nm of size) with 4.7 × 10⁶ M⁻¹ of the stability constant in the physiological media. The acidity and ion strength of the media obviously affected the binding of lysozyme. The warping and deformation of the lysozyme bridging were demonstrated by the conversion of its spatial structure from α-helix into a β-sheet, measured by CD. In the presence of nano-TiO₂, the bacteriolysis activity of lysozyme was subjected to an obvious inhibition. Discussion The two-step binding model of lysozyme was proposed, in which lysozyme was adsorbed on nano-TiO₂ particle surface by electrostatic interaction and then the hydrogen bond (N-H···O and O-H···O) formed between nano-TiO₂ particle and polar side groups of lysozyme. The adsorption of lysozyme obeyed the Langmuir isothermal model. The binding of lysozyme is dependent on the acidity and ion strength of the media. The bigger TiO₂ aggregate was formed in the presence of lysozyme where lysozyme may bridge between nano-TiO₂ particles. The coexistence of nano-TiO₂ particles resulted in the transition of lysozyme conformation from an α-helix into a β-sheet and a substantial inactivation of lysozyme. The β-sheet can induce the formation of amyloid fibrils, a process which plays a major role in |
| doi_str_mv | 10.1007/s11356-009-0153-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_220577358</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1968702611</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-95851ac332028ea93712dfef7a6f8d93765375c205ee996e962295c1cd22d1cc3</originalsourceid><addsrcrecordid>eNp9kMFOGzEQhq0KVALtA_RCrd5dZuz1rt0bQqUgIXEonC3j9SZGyTrYjtpw5FF5kjraVJzak8ea__tH-gj5hPAVAbqzjChkywA0A5SC4TsywxYb1jVaH5AZ6KZhKJrmiBzn_AjAQfPuPTlCLTQopWbk4XosPllXQhxpHOhox8juwu3rywv9FcqCLrc5Pm9X_hsNYw7zRcl1KJGWhad-3G1oib-DC2X7l8_h2fd0bVMJbunzB3I42GX2H_fvCbm__H53ccVubn9cX5zfMNegKkxLJdE6IThw5a0WHfJ-8ENn20H19dtK0UnHQXqvdet1y7mWDl3PeY_OiRPyZepdp_i08bmYx7hJYz1peKW6TkhVQziFXIo5Jz-YdQorm7YGweykmkmqqVLNTqrBypzuizcPK9-_EXuLNcCnQK6rce7T2-X_tX6eoMFGY-cpZHP_kwM2ANAi1-qfCQGooOVKiD_q8pVZ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>220577358</pqid></control><display><type>article</type><title>Interaction of nano-TiO₂ with lysozyme: insights into the enzyme toxicity of nanosized particles</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Xu, Zhen ; Liu, Xi-Wei ; Ma, Yin-Sheng ; Gao, Hong-Wen</creator><creatorcontrib>Xu, Zhen ; Liu, Xi-Wei ; Ma, Yin-Sheng ; Gao, Hong-Wen</creatorcontrib><description>Background, aim, and scope Nanomaterials have been used increasingly in industrial production and daily life, but their human exposure may cause health risks. The interactions of nanomaterial with functional biomolecules are often applied as a precondition for its cytotoxicity and organ toxicity where various proteins have been investigated in the past years. In the present study, nano-TiO₂ was selected as the representative of nanomaterials and lysozyme as a representative for enzymes. By investigating their interaction by various instrumentations, the objective is to identify the action sites and types, estimate the effect on the enzyme structure and activity, and reveal the toxicity mechanism of nanomaterial. Materials and methods Laboratory-scale experiments were carried out to investigate the interactions of nano-TiO₂ with lysozyme. The interaction of nano-TiO₂ particles with lysozyme has been studied in the analogous physiological media in detail by UV spectrometry, fluorophotometry, circular dichroism (CD), scanning electron microscope, ζ-potential, and laser particle size. Results The interaction accorded with the Langmuir isothermal adsorption and the saturation number of lysozyme is determined to be 580 per nano-TiO₂ particle (60 nm of size) with 4.7 × 10⁶ M⁻¹ of the stability constant in the physiological media. The acidity and ion strength of the media obviously affected the binding of lysozyme. The warping and deformation of the lysozyme bridging were demonstrated by the conversion of its spatial structure from α-helix into a β-sheet, measured by CD. In the presence of nano-TiO₂, the bacteriolysis activity of lysozyme was subjected to an obvious inhibition. Discussion The two-step binding model of lysozyme was proposed, in which lysozyme was adsorbed on nano-TiO₂ particle surface by electrostatic interaction and then the hydrogen bond (N-H···O and O-H···O) formed between nano-TiO₂ particle and polar side groups of lysozyme. The adsorption of lysozyme obeyed the Langmuir isothermal model. The binding of lysozyme is dependent on the acidity and ion strength of the media. The bigger TiO₂ aggregate was formed in the presence of lysozyme where lysozyme may bridge between nano-TiO₂ particles. The coexistence of nano-TiO₂ particles resulted in the transition of lysozyme conformation from an α-helix into a β-sheet and a substantial inactivation of lysozyme. The β-sheet can induce the formation of amyloid fibrils, a process which plays a major role in pathology. Conclusions Lysozyme was adsorbed on the nano-TiO₂ particle surface via electrostatic attraction and hydrogen bonds, and they also bridged among global nano-TiO₂ particles to form the colloidal particles. As a reasonable deduction of this study, nano-TiO₂ might have some toxic impacts on biomolecules. Our data suggest that careful attention be paid to the interaction of protein and nanomaterials. This could contribute to nanomaterial toxicity assessment. Recommendations and perspectives Our results strongly suggest that nano-TiO₂ has an obvious impact on biomolecules. Our data suggest that more attention should be paid to the potential toxicity of nano-TiO₂ on biomolecules. Further research into the toxicity of nanosized particles needs to be carried out prior to their cell toxicity and tissue toxicity. These investigations might serve as the basis for determining the toxicity and application of nanomaterials.</description><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-009-0153-1</identifier><identifier>PMID: 19390888</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Acidity ; Adsorption ; Amyloid ; Aquatic Pollution ; AREA 7.2 • Interactions of Chemicals • RESEARCH ARTICLE ; Atmospheric Protection/Air Quality Control/Air Pollution ; Circular dichroism ; Cytotoxicity ; deformation ; DNA damage ; Earth and Environmental Science ; Ecotoxicology ; electrostatic interactions ; Electrostatic properties ; Environment ; Environmental Chemistry ; Environmental Health ; Enzymes ; Fibrillogenesis ; Health risks ; Human exposure ; Hydrogen bonding ; Hydrogen-Ion Concentration ; Inactivation ; Industrial production ; Investigations ; Laboratories ; Lysozyme ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - toxicity ; Metal Nanoparticles - ultrastructure ; Muramidase - chemistry ; Muramidase - drug effects ; Muramidase - ultrastructure ; Nanomaterials ; Nanotechnology ; Osmolar Concentration ; Particle size ; Physiology ; Protein Conformation - drug effects ; Protein Structure, Secondary - drug effects ; Proteins ; Quantum dots ; risk ; Risk assessment ; scanning electron microscopes ; Scanning electron microscopy ; Spectrometry ; Studies ; Temperature ; Titanium - chemistry ; Titanium - toxicity ; Titanium dioxide ; Toxicity ; Toxicity Tests ; ultraviolet-visible spectroscopy ; Waste Water Technology ; Water Management ; Water Pollution Control ; Zeta potential</subject><ispartof>Environmental science and pollution research international, 2010-03, Vol.17 (3), p.798-806</ispartof><rights>Springer-Verlag 2009</rights><rights>Springer-Verlag 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-95851ac332028ea93712dfef7a6f8d93765375c205ee996e962295c1cd22d1cc3</citedby><cites>FETCH-LOGICAL-c418t-95851ac332028ea93712dfef7a6f8d93765375c205ee996e962295c1cd22d1cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11356-009-0153-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11356-009-0153-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19390888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Zhen</creatorcontrib><creatorcontrib>Liu, Xi-Wei</creatorcontrib><creatorcontrib>Ma, Yin-Sheng</creatorcontrib><creatorcontrib>Gao, Hong-Wen</creatorcontrib><title>Interaction of nano-TiO₂ with lysozyme: insights into the enzyme toxicity of nanosized particles</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>Background, aim, and scope Nanomaterials have been used increasingly in industrial production and daily life, but their human exposure may cause health risks. The interactions of nanomaterial with functional biomolecules are often applied as a precondition for its cytotoxicity and organ toxicity where various proteins have been investigated in the past years. In the present study, nano-TiO₂ was selected as the representative of nanomaterials and lysozyme as a representative for enzymes. By investigating their interaction by various instrumentations, the objective is to identify the action sites and types, estimate the effect on the enzyme structure and activity, and reveal the toxicity mechanism of nanomaterial. Materials and methods Laboratory-scale experiments were carried out to investigate the interactions of nano-TiO₂ with lysozyme. The interaction of nano-TiO₂ particles with lysozyme has been studied in the analogous physiological media in detail by UV spectrometry, fluorophotometry, circular dichroism (CD), scanning electron microscope, ζ-potential, and laser particle size. Results The interaction accorded with the Langmuir isothermal adsorption and the saturation number of lysozyme is determined to be 580 per nano-TiO₂ particle (60 nm of size) with 4.7 × 10⁶ M⁻¹ of the stability constant in the physiological media. The acidity and ion strength of the media obviously affected the binding of lysozyme. The warping and deformation of the lysozyme bridging were demonstrated by the conversion of its spatial structure from α-helix into a β-sheet, measured by CD. In the presence of nano-TiO₂, the bacteriolysis activity of lysozyme was subjected to an obvious inhibition. Discussion The two-step binding model of lysozyme was proposed, in which lysozyme was adsorbed on nano-TiO₂ particle surface by electrostatic interaction and then the hydrogen bond (N-H···O and O-H···O) formed between nano-TiO₂ particle and polar side groups of lysozyme. The adsorption of lysozyme obeyed the Langmuir isothermal model. The binding of lysozyme is dependent on the acidity and ion strength of the media. The bigger TiO₂ aggregate was formed in the presence of lysozyme where lysozyme may bridge between nano-TiO₂ particles. The coexistence of nano-TiO₂ particles resulted in the transition of lysozyme conformation from an α-helix into a β-sheet and a substantial inactivation of lysozyme. The β-sheet can induce the formation of amyloid fibrils, a process which plays a major role in pathology. Conclusions Lysozyme was adsorbed on the nano-TiO₂ particle surface via electrostatic attraction and hydrogen bonds, and they also bridged among global nano-TiO₂ particles to form the colloidal particles. As a reasonable deduction of this study, nano-TiO₂ might have some toxic impacts on biomolecules. Our data suggest that careful attention be paid to the interaction of protein and nanomaterials. This could contribute to nanomaterial toxicity assessment. Recommendations and perspectives Our results strongly suggest that nano-TiO₂ has an obvious impact on biomolecules. Our data suggest that more attention should be paid to the potential toxicity of nano-TiO₂ on biomolecules. Further research into the toxicity of nanosized particles needs to be carried out prior to their cell toxicity and tissue toxicity. These investigations might serve as the basis for determining the toxicity and application of nanomaterials.</description><subject>Acidity</subject><subject>Adsorption</subject><subject>Amyloid</subject><subject>Aquatic Pollution</subject><subject>AREA 7.2 • Interactions of Chemicals • RESEARCH ARTICLE</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Circular dichroism</subject><subject>Cytotoxicity</subject><subject>deformation</subject><subject>DNA damage</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>electrostatic interactions</subject><subject>Electrostatic properties</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Enzymes</subject><subject>Fibrillogenesis</subject><subject>Health risks</subject><subject>Human exposure</subject><subject>Hydrogen bonding</subject><subject>Hydrogen-Ion Concentration</subject><subject>Inactivation</subject><subject>Industrial production</subject><subject>Investigations</subject><subject>Laboratories</subject><subject>Lysozyme</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - toxicity</subject><subject>Metal Nanoparticles - ultrastructure</subject><subject>Muramidase - chemistry</subject><subject>Muramidase - drug effects</subject><subject>Muramidase - ultrastructure</subject><subject>Nanomaterials</subject><subject>Nanotechnology</subject><subject>Osmolar Concentration</subject><subject>Particle size</subject><subject>Physiology</subject><subject>Protein Conformation - drug effects</subject><subject>Protein Structure, Secondary - drug effects</subject><subject>Proteins</subject><subject>Quantum dots</subject><subject>risk</subject><subject>Risk assessment</subject><subject>scanning electron microscopes</subject><subject>Scanning electron microscopy</subject><subject>Spectrometry</subject><subject>Studies</subject><subject>Temperature</subject><subject>Titanium - chemistry</subject><subject>Titanium - toxicity</subject><subject>Titanium dioxide</subject><subject>Toxicity</subject><subject>Toxicity Tests</subject><subject>ultraviolet-visible spectroscopy</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><subject>Zeta 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of nano-TiO₂ with lysozyme: insights into the enzyme toxicity of nanosized particles</title><author>Xu, Zhen ; Liu, Xi-Wei ; Ma, Yin-Sheng ; Gao, Hong-Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-95851ac332028ea93712dfef7a6f8d93765375c205ee996e962295c1cd22d1cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acidity</topic><topic>Adsorption</topic><topic>Amyloid</topic><topic>Aquatic Pollution</topic><topic>AREA 7.2 • Interactions of Chemicals • RESEARCH ARTICLE</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Circular dichroism</topic><topic>Cytotoxicity</topic><topic>deformation</topic><topic>DNA damage</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>electrostatic interactions</topic><topic>Electrostatic properties</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Enzymes</topic><topic>Fibrillogenesis</topic><topic>Health risks</topic><topic>Human exposure</topic><topic>Hydrogen bonding</topic><topic>Hydrogen-Ion Concentration</topic><topic>Inactivation</topic><topic>Industrial production</topic><topic>Investigations</topic><topic>Laboratories</topic><topic>Lysozyme</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metal Nanoparticles - toxicity</topic><topic>Metal Nanoparticles - ultrastructure</topic><topic>Muramidase - chemistry</topic><topic>Muramidase - drug effects</topic><topic>Muramidase - ultrastructure</topic><topic>Nanomaterials</topic><topic>Nanotechnology</topic><topic>Osmolar Concentration</topic><topic>Particle size</topic><topic>Physiology</topic><topic>Protein Conformation - drug effects</topic><topic>Protein Structure, Secondary - drug effects</topic><topic>Proteins</topic><topic>Quantum dots</topic><topic>risk</topic><topic>Risk assessment</topic><topic>scanning electron microscopes</topic><topic>Scanning electron microscopy</topic><topic>Spectrometry</topic><topic>Studies</topic><topic>Temperature</topic><topic>Titanium - chemistry</topic><topic>Titanium - toxicity</topic><topic>Titanium dioxide</topic><topic>Toxicity</topic><topic>Toxicity Tests</topic><topic>ultraviolet-visible spectroscopy</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><topic>Zeta potential</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Zhen</creatorcontrib><creatorcontrib>Liu, Xi-Wei</creatorcontrib><creatorcontrib>Ma, Yin-Sheng</creatorcontrib><creatorcontrib>Gao, Hong-Wen</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE 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Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Environmental science and pollution research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Zhen</au><au>Liu, Xi-Wei</au><au>Ma, Yin-Sheng</au><au>Gao, Hong-Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of nano-TiO₂ with lysozyme: insights into the enzyme toxicity of nanosized particles</atitle><jtitle>Environmental science and pollution research international</jtitle><stitle>Environ Sci Pollut Res</stitle><addtitle>Environ Sci Pollut Res Int</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>17</volume><issue>3</issue><spage>798</spage><epage>806</epage><pages>798-806</pages><issn>0944-1344</issn><eissn>1614-7499</eissn><abstract>Background, aim, and scope Nanomaterials have been used increasingly in industrial production and daily life, but their human exposure may cause health risks. The interactions of nanomaterial with functional biomolecules are often applied as a precondition for its cytotoxicity and organ toxicity where various proteins have been investigated in the past years. In the present study, nano-TiO₂ was selected as the representative of nanomaterials and lysozyme as a representative for enzymes. By investigating their interaction by various instrumentations, the objective is to identify the action sites and types, estimate the effect on the enzyme structure and activity, and reveal the toxicity mechanism of nanomaterial. Materials and methods Laboratory-scale experiments were carried out to investigate the interactions of nano-TiO₂ with lysozyme. The interaction of nano-TiO₂ particles with lysozyme has been studied in the analogous physiological media in detail by UV spectrometry, fluorophotometry, circular dichroism (CD), scanning electron microscope, ζ-potential, and laser particle size. Results The interaction accorded with the Langmuir isothermal adsorption and the saturation number of lysozyme is determined to be 580 per nano-TiO₂ particle (60 nm of size) with 4.7 × 10⁶ M⁻¹ of the stability constant in the physiological media. The acidity and ion strength of the media obviously affected the binding of lysozyme. The warping and deformation of the lysozyme bridging were demonstrated by the conversion of its spatial structure from α-helix into a β-sheet, measured by CD. In the presence of nano-TiO₂, the bacteriolysis activity of lysozyme was subjected to an obvious inhibition. Discussion The two-step binding model of lysozyme was proposed, in which lysozyme was adsorbed on nano-TiO₂ particle surface by electrostatic interaction and then the hydrogen bond (N-H···O and O-H···O) formed between nano-TiO₂ particle and polar side groups of lysozyme. The adsorption of lysozyme obeyed the Langmuir isothermal model. The binding of lysozyme is dependent on the acidity and ion strength of the media. The bigger TiO₂ aggregate was formed in the presence of lysozyme where lysozyme may bridge between nano-TiO₂ particles. The coexistence of nano-TiO₂ particles resulted in the transition of lysozyme conformation from an α-helix into a β-sheet and a substantial inactivation of lysozyme. The β-sheet can induce the formation of amyloid fibrils, a process which plays a major role in pathology. Conclusions Lysozyme was adsorbed on the nano-TiO₂ particle surface via electrostatic attraction and hydrogen bonds, and they also bridged among global nano-TiO₂ particles to form the colloidal particles. As a reasonable deduction of this study, nano-TiO₂ might have some toxic impacts on biomolecules. Our data suggest that careful attention be paid to the interaction of protein and nanomaterials. This could contribute to nanomaterial toxicity assessment. Recommendations and perspectives Our results strongly suggest that nano-TiO₂ has an obvious impact on biomolecules. Our data suggest that more attention should be paid to the potential toxicity of nano-TiO₂ on biomolecules. Further research into the toxicity of nanosized particles needs to be carried out prior to their cell toxicity and tissue toxicity. These investigations might serve as the basis for determining the toxicity and application of nanomaterials.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19390888</pmid><doi>10.1007/s11356-009-0153-1</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0944-1344 |
| ispartof | Environmental science and pollution research international, 2010-03, Vol.17 (3), p.798-806 |
| issn | 0944-1344 1614-7499 |
| language | eng |
| recordid | cdi_proquest_journals_220577358 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Acidity Adsorption Amyloid Aquatic Pollution AREA 7.2 • Interactions of Chemicals • RESEARCH ARTICLE Atmospheric Protection/Air Quality Control/Air Pollution Circular dichroism Cytotoxicity deformation DNA damage Earth and Environmental Science Ecotoxicology electrostatic interactions Electrostatic properties Environment Environmental Chemistry Environmental Health Enzymes Fibrillogenesis Health risks Human exposure Hydrogen bonding Hydrogen-Ion Concentration Inactivation Industrial production Investigations Laboratories Lysozyme Metal Nanoparticles - chemistry Metal Nanoparticles - toxicity Metal Nanoparticles - ultrastructure Muramidase - chemistry Muramidase - drug effects Muramidase - ultrastructure Nanomaterials Nanotechnology Osmolar Concentration Particle size Physiology Protein Conformation - drug effects Protein Structure, Secondary - drug effects Proteins Quantum dots risk Risk assessment scanning electron microscopes Scanning electron microscopy Spectrometry Studies Temperature Titanium - chemistry Titanium - toxicity Titanium dioxide Toxicity Toxicity Tests ultraviolet-visible spectroscopy Waste Water Technology Water Management Water Pollution Control Zeta potential |
| title | Interaction of nano-TiO₂ with lysozyme: insights into the enzyme toxicity of nanosized particles |
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