Development of an Antibody Hapten-Chip System for Detecting the Residues of Multiple Antibiotic Drugs

:  The abuse of antibiotic drugs during animal production remains a worldwide problem and the subsequent detection of the residues of various drugs present at low concentrations in complex biological matrices poses significant analytical challenges. The present study outlines a practical biochip ass...

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Veröffentlicht in:Journal of forensic sciences 2009-07, Vol.54 (4), p.953-960
Hauptverfasser: Chen, Ailiang, Wang, Guoqing, Cao, Qin, Wang, Yan, Zhang, Zhaohui, Sun, Yimin, Wang, Hui, Xu, Chaoyi, Zhou, Qi, Han, Pei, Liu, Miao, Yang, Yang, Xing, Wanli, Mitchelson, Keith R., Cheng, Jing
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Sprache:eng
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Zusammenfassung::  The abuse of antibiotic drugs during animal production remains a worldwide problem and the subsequent detection of the residues of various drugs present at low concentrations in complex biological matrices poses significant analytical challenges. The present study outlines a practical biochip assay system to identify antibiotic residues in different animal tissue extracts. The system uses a simple but efficient multiresidue sample extraction procedure to isolate the antibiotic residues which were then identified directly using high‐affinity monoclonal antibodies presented in a competitive immunoassay with conjugated antibiotic hapten‐chips. The hapten‐chip can analyze six samples each for eight antibiotics on a single chip within 3 h. The analytical results with both artificial positive standard samples and the incurred samples show that the antibody hapten‐chip system has a comparable accuracy and a similar sensitivity to a standard ultra performance liquid chromatography–mass spectrometry (MS)/MS assay. In conclusion, an effective analytical screening system based on antibody hapten‐chip was developed for detecting multiple antibiotic residues from multiple samples.
ISSN:0022-1198
1556-4029
DOI:10.1111/j.1556-4029.2009.01049.x