Chemical constituents from Polyalthia longifolia seeds extract by gas Chromatography-Mass spectroscopy (GC-MS) studies

Materials and Methods:Present research studies encompasses identification of chemical constituents in PL seeds petroleum ether extracts by hyphenated techniques such as Gas Chromatography -Mass Spectroscopy (GC-MS) and identified by matching mass spectra with mass spectrum libraries.Results: Extraction: * Fresh seeds, free from any visible contamination were collected and air dried under normal environmental condition and homogenized to coarse powder. * 200 g of grinded seeds were then soaked and macerated in 1000 ml of petroleum ether for 7 days at room temperature. * Extracts were then filtered by using Whatman filter paper no.1. * Extracts were concentrated and stored in the refrigerator for future use. Gas Chromatography-Mass spectroscopy: * The instrument used in the experimentation purpose was Joel, USAwith model of Accu Time of Flight GCV. * The column details comprises ofCapillary (Type), Semistandard nonpolar (Class), 30m/60m(length) with diameter of 0.25 mm. * The mobile phase waspetroleum ether and the Carrier used was Helium with heat rate of2k/min to 3k/min. * The libraries used wereNIST 2.0 f and Fine, NIH, EINECS, TSCA, RTECS, HODOC, IRDB, LIB for identification and interpretation of compounds. In the current research article, PL seed petroleum ether extract shows the presence of distinct bioactive compounds which can be used for treatment of various disorders in the future.In addition, research can be elaborated and focused on numerous different extraction procedures and elucidation and comparison of various phytoconstituents by application of various chromatographic hyphenated techniques can be undertaken.