Isolation, Characterization and Sequencing of Lactobacillus from the Oral and Fecal Samples of Healthy Dogs

[...]the growth inhibition factors were determined by the formation of the zone around the point of the pellet. [...]this determines the pro-biotic effect of the lactobacillus species over other pathological microorganisms. The catalase test was found to be Negative. Since anaerobic Organisms do not produce catalase enzyme for the degradation of hydrogen peroxide, the obtained organism is anaerobic. .The citrate test for the obtained organisms were found to be negative. The samples were able to withstand low pH conditions and they could tolerate bile and sodium chloride concentrations. [...]the Biochemical characterization of those species was found to be lactobacillus species. The DNA from the obtained species were separated, amplified and then sent for sequencing and to match them with the sequence database as to determine the organism obtained. [...]the sequence similarity levels with Lactobacillus casei is 93% followed by 89% with Lactobacillus pentosus.