Enantioseparation of Metoprolol Tartrate using HPLC by Adding Methyl beta Cyclodextrin to the mobile Phase (As Chiral Additive)

Chromatographic separation was conducted in the reversed phase operating mode, with a mobile phase consists of aqueous solution contains chiral selector Mβ-CD (1.5 g/l)/methanol with the volumetric ratio of 86:14 respectively , (aqueous solution :add to one liter of HPLC water 3.3 ml TEA the adjust the pH on 3.1 using glacial acetic acid), The final pH value of mobile phase was adjusted by using glacial acetic acid. The measurements were carried out in an isocratic elution mode at ambient temperature with a flow rate of 0.5 mL/min, an injection volume of 20 pL and UV detection at 274 nm. The most suitable mobile phase composition for enantiomeric resolution of metoprolol tartrate is (aqueous solution :add to one liter of HPLC water 3.3 ml TEA the adjust the pH on 3.1 using glacial acetic acid) 86%: methanol 16%) and the other conditions are (M-ßCD 1.5 g/l ,pH of 3.1, flow rate 0.5 ml/min, detection wavelength at 274nm, at room temperature) as shown in table 1. Tables 3 shows the effect on the USP resolution(Rs) of variation in final pH value of mobile phase and the other conditions are (M -ß-CD 1.5 g/l, flow rate 0.5 ml/min, detection wavelength at 274nm, at room temperature).