Extraction, Isolation and Analysis of Chondroitin Sulphate from Chicken Shank by Spectrophotometric Method

First group was extracted by incubation at pH 4.5 using 20 mL acetic acid 10% and temperature of 37°C for 6h. [...]the method provides good precision and reproducibility.11 Detection limit: [...]the method provided good precision. [...]the method provided good precision and reproducibility. CS purity from chicken shank cartilage was 87.87% for 8h extraction by (acid hydrolysis extraction method) whilst certificate of analysis of the CS from Fenchem Biotek Ltd stated 90.47% and those from Chemical Point UG Germany stated 90.7%. [...]the method needs more purification steps.