Validated Stability Indicating RP-HPLC method for estimation of antiviral class of drugs Sofosbuvir and Velpatasvir in combination and its comparison with reported methods

Velpatasvir is a direct acting antiviral (DAA) medication used as part of combination therapy to treat chronic Hepatitis C, an infectious liver disease caused by infection with Hepatitis C Virus (HCV). [...]the implementation of an analytical methodology to determine sofosbuvir and velpatasvir simultaneously, in presence of its degradation products is rather a challenge for pharmaceutical analyst. [...]it was thought necessary to study the stability of sofosbuvir and velpatasvir under acidic, alkaline, oxidative, UV and photolytic conditions. The HPLC analysis was performed on the above mentioned chromatographic system by using isocratic elution mode using a mobile phase of 0.1% OPA Buffer:Acetonitrile (50:50, v/v) on BDS C18 column (250·4.6 mm, 5 pm particle size) with 1 ml/min flow rate at 240 nm using photodiode array detector by maintain ambient temperature. A novel treatment option for chronic hepatitis C infection, Journal of Pharmacology and Pharmacotherapeutics. 2014; 5(4): 278-284.