In   vitro Anti-Cancer Study of Vitis   viniferae , Ixora   coccinea and Piper   longum Extract on Human Breast Carcinoma Cells

Here, we investigated the anti-cancer effects of 3 drug extracts-vitis viniferae-Grape seed (GSE), Ixora coccinea flower and Piper longum root extract against breast cancer treatment. cells was cultured in DMEM supplemented with 10% inactivated Fetal Bovine Serum (FBS), 1% penicillin in an humidified atmosphere of 5% CO2 at 37 °C. The IC50 of a drug can be determined by constructing a dose-response curve.The percentage inhibition of vitis viniferae (10-320 pg/ml),Ixora coccinea (10-320 pg/ml) and Piper longum (10-320 pg/ml) treatment of cells resulted in 10-90%, 6-53% and 14-90% growth inhibition respectively.Cell proliferation decreased in a concentration and time-dependent manner. [...]results showed that 3 drug extracts exhibits cytotoxic activity to MCF-7 cells and it could be a potential anticancer drug. Mammary cancers exhibit heterogeneity not only with respect to hormones- oestrogen, progesterone and receptors -human epidermal growth factor-2 (HER-2) expression but also with tumour size, grade and nodal status,hence different treatment is needed(3). [...]breast cancer is a diverse phenotype mixture, for which different treatment needed and there is no cure for breast cancer at the present moment(4), thus new drug search having lesser side effects such as herbal remedies is needed to treat and to control the disease. Procyanidin B3, an inhibitor of histone acetyltransferase, enhances the action of antagonist for prostate cancer cells via inhibition of p300-dependent acetylation of androgen receptor.