An enhancement of luminol chemiluminescence by cobalt hydroxide decorated porous graphene and its application in glucose analysis

Here, it was shown that a cobalt hydroxide/porous-graphene nanocomposite (PG/Co(OH) 2 ) has great catalytic capability toward luminol as a chemiluminescence active substrate. We made use of PG/Co(OH) 2 for developing a super-sensitive quantitative analytical tool for glucose detection. After optimizing the effective factors, the relative chemiluminescence intensity was found to linearly change with H 2 O 2 concentration from 5.0 × 10 −11 to 1.0 × 10 −6 mol L −1 ( R 2 = 0.99023) and a detection limit as low as 8.0 × 10 −12 mol L −1 (S/N = 3). The association between the CL signal intensity and H 2 O 2 concentration was obtained. Based on the production of H 2 O 2 by glucose oxidase from glucose, the detection of glucose was performed and it could be linearly detected in the range from 3.0 × 10 −9 to 4.0 × 10 −5 mol L −1 ( R 2 = 0.99106) with a detection limit of 6.4 × 10 −10 mol L −1 . This method was also implemented for non-invasive determination of glucose in tears and saliva, which is important for diabetic patients with frequent/continuous glucose monitoring requirements, and satisfactory results were obtained. Here, it was shown that a cobalt hydroxide/porous-graphene nanocomposite (PG/Co(OH) 2 ) has great catalytic capability toward luminol as a chemiluminescence active substrate.