Evaluation of inhibitory effect of coptisine on protein kinase C activity using a RI detection-assisted biochip

In this study, a radioisotope (RI) detection-assisted biochip was developed for the evaluation of the inhibitory effects of natural products on protein kinase C (PKC) activity. It was shown that PKC activity was inhibited by coptisine in dose-dependent manner. These findings suggest that the RI dete...

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Veröffentlicht in:	Journal of radioanalytical and nuclear chemistry 2019-03, Vol.319 (3), p.1103-1110
Hauptverfasser:	Kang, Jung Ae, Rho, Jong Kook, Park, Sang Hyun
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochips Chemical compounds Chemistry Chemistry and Materials Science Diagnostic Radiology Energy industries Hadrons Heavy Ions Inhibitors Inorganic Chemistry Kinases Natural products Nuclear Chemistry Nuclear energy Nuclear Physics Pharmacology Physical Chemistry Protein kinases Proteins Radiation (Physics) Radioisotopes Technical institutes Technology
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Beschreibung
Zusammenfassung:	In this study, a radioisotope (RI) detection-assisted biochip was developed for the evaluation of the inhibitory effects of natural products on protein kinase C (PKC) activity. It was shown that PKC activity was inhibited by coptisine in dose-dependent manner. These findings suggest that the RI detection-assisted protein biochip should be applicable to high throughput screening for kinase inhibitors. It was found that coptisine shows inhibitory effect on PKC, and that the biochip can be used to rapidly screen kinase inhibitors of therapeutic agents.
ISSN:	0236-5731 1588-2780
DOI:	10.1007/s10967-018-06410-8