DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins1[C][W][OA]
LONG HYPOCOTYL5 (HY5) is a bZIP (basic leucine zipper) transcription factor that activates photomorphogenesis and root development in Arabidopsis (Arabidopsis thaliana). Previously, STF1 (soybean [Glycine max] TGACG-motif binding factor 1), a homologous legume protein with a RING-finger motif and a...
Gespeichert in:
Veröffentlicht in: | Plant physiology (Bethesda) 2008-04, Vol.146 (4), p.1862 |
---|---|
Hauptverfasser: | , , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | 4 |
container_start_page | 1862 |
container_title | Plant physiology (Bethesda) |
container_volume | 146 |
creator | Song, Young Hun Yoo, Cheol Min Hong, An Pio Kim, Seong Hee Jeong, Hee Jeong Shin, Su Young Kim, Hye Jin Yun, Dae-Jin Lim, Chae Oh Bahk, Jeong Dong Lee, Sang Yeol Nagao, Ron T Key, Joe L Hong, Jong Chan |
description | LONG HYPOCOTYL5 (HY5) is a bZIP (basic leucine zipper) transcription factor that activates photomorphogenesis and root development in Arabidopsis (Arabidopsis thaliana). Previously, STF1 (soybean [Glycine max] TGACG-motif binding factor 1), a homologous legume protein with a RING-finger motif and a bZIP domain, was reported in soybean. To investigate the role of STF1, the phenotypes of transgenic Arabidopsis plants overexpressing STF1 and HY5 were compared. In addition, the DNA-binding properties of STF1 and HY5 were extensively studied using random binding site selection and electrophoretic mobility shift assay. Overexpression of STF1 in the hy5 mutant of Arabidopsis restored wild-type photomorphogenic and root development phenotypes of short hypocotyl, accumulation of chlorophyll, and root gravitropism with partial restoration of anthocyanin accumulation. This supports that STF1 is a homolog of HY5 with a role in light and hormone signaling. The DNA-binding properties of STF1 and HY5 are shown to be similar to each other in recognizing many ACGT-containing elements with a consensus sequence motif of 5'-(G/A)(G/A) TGACGT(C/G/A)(A/T/G)-3'. The motif represents a characteristically strong preference for flanking sequence to TGACGT and a larger sequence than the sequences recognized by the G-box binding factor and TGA protein families. The finding of C-box, hybrid C/G-, and C/A-boxes as high-affinity binding sites over the G-box and parameters associated with HY5 recognition define the criteria of HY5/STF1 protein-DNA interaction in the promoter regions. This study helps to predict the precise in vivo binding sites of the HY5 protein from the vast number of putative HY5 genomic binding sites analyzed by chromatin immunoprecipitation on chip. |
format | Article |
fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_218586155</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1484840411</sourcerecordid><originalsourceid>FETCH-proquest_journals_2185861553</originalsourceid><addsrcrecordid>eNqNjF1LwzAUhoMoWD_-w8H7YNIuEi-76FZhroMVZJQyKklmhiSapGB_iX_XUMRrr87Ded_3OUEZZUWOczbjpygjJDHh_P4cXYRwJITQgs4y9P2wLvHcWGnsAbZxkCM8SWWj0UYFEHjuvqC3Eqrx1RsJ4nY5vZxPWE747FI5QB-gMoc3XGptrIkj_ElNTCadFttmQSfZql4vodptalE3uxWDjXdRGRtoK7r2pWvrsrtCZ7p_D-r6916im8VjIyr84d3noELcH93gbYr2OeWM31HGin-VfgBOkFQv</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>218586155</pqid></control><display><type>article</type><title>DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins1[C][W][OA]</title><source>Jstor Complete Legacy</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Song, Young Hun ; Yoo, Cheol Min ; Hong, An Pio ; Kim, Seong Hee ; Jeong, Hee Jeong ; Shin, Su Young ; Kim, Hye Jin ; Yun, Dae-Jin ; Lim, Chae Oh ; Bahk, Jeong Dong ; Lee, Sang Yeol ; Nagao, Ron T ; Key, Joe L ; Hong, Jong Chan</creator><creatorcontrib>Song, Young Hun ; Yoo, Cheol Min ; Hong, An Pio ; Kim, Seong Hee ; Jeong, Hee Jeong ; Shin, Su Young ; Kim, Hye Jin ; Yun, Dae-Jin ; Lim, Chae Oh ; Bahk, Jeong Dong ; Lee, Sang Yeol ; Nagao, Ron T ; Key, Joe L ; Hong, Jong Chan</creatorcontrib><description>LONG HYPOCOTYL5 (HY5) is a bZIP (basic leucine zipper) transcription factor that activates photomorphogenesis and root development in Arabidopsis (Arabidopsis thaliana). Previously, STF1 (soybean [Glycine max] TGACG-motif binding factor 1), a homologous legume protein with a RING-finger motif and a bZIP domain, was reported in soybean. To investigate the role of STF1, the phenotypes of transgenic Arabidopsis plants overexpressing STF1 and HY5 were compared. In addition, the DNA-binding properties of STF1 and HY5 were extensively studied using random binding site selection and electrophoretic mobility shift assay. Overexpression of STF1 in the hy5 mutant of Arabidopsis restored wild-type photomorphogenic and root development phenotypes of short hypocotyl, accumulation of chlorophyll, and root gravitropism with partial restoration of anthocyanin accumulation. This supports that STF1 is a homolog of HY5 with a role in light and hormone signaling. The DNA-binding properties of STF1 and HY5 are shown to be similar to each other in recognizing many ACGT-containing elements with a consensus sequence motif of 5'-(G/A)(G/A) TGACGT(C/G/A)(A/T/G)-3'. The motif represents a characteristically strong preference for flanking sequence to TGACGT and a larger sequence than the sequences recognized by the G-box binding factor and TGA protein families. The finding of C-box, hybrid C/G-, and C/A-boxes as high-affinity binding sites over the G-box and parameters associated with HY5 recognition define the criteria of HY5/STF1 protein-DNA interaction in the promoter regions. This study helps to predict the precise in vivo binding sites of the HY5 protein from the vast number of putative HY5 genomic binding sites analyzed by chromatin immunoprecipitation on chip.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><language>eng</language><publisher>Rockville: American Society of Plant Biologists</publisher><subject>Deoxyribonucleic acid ; DNA ; Root development ; Site selection ; Soybeans</subject><ispartof>Plant physiology (Bethesda), 2008-04, Vol.146 (4), p.1862</ispartof><rights>Copyright American Society of Plant Biologists Apr 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Song, Young Hun</creatorcontrib><creatorcontrib>Yoo, Cheol Min</creatorcontrib><creatorcontrib>Hong, An Pio</creatorcontrib><creatorcontrib>Kim, Seong Hee</creatorcontrib><creatorcontrib>Jeong, Hee Jeong</creatorcontrib><creatorcontrib>Shin, Su Young</creatorcontrib><creatorcontrib>Kim, Hye Jin</creatorcontrib><creatorcontrib>Yun, Dae-Jin</creatorcontrib><creatorcontrib>Lim, Chae Oh</creatorcontrib><creatorcontrib>Bahk, Jeong Dong</creatorcontrib><creatorcontrib>Lee, Sang Yeol</creatorcontrib><creatorcontrib>Nagao, Ron T</creatorcontrib><creatorcontrib>Key, Joe L</creatorcontrib><creatorcontrib>Hong, Jong Chan</creatorcontrib><title>DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins1[C][W][OA]</title><title>Plant physiology (Bethesda)</title><description>LONG HYPOCOTYL5 (HY5) is a bZIP (basic leucine zipper) transcription factor that activates photomorphogenesis and root development in Arabidopsis (Arabidopsis thaliana). Previously, STF1 (soybean [Glycine max] TGACG-motif binding factor 1), a homologous legume protein with a RING-finger motif and a bZIP domain, was reported in soybean. To investigate the role of STF1, the phenotypes of transgenic Arabidopsis plants overexpressing STF1 and HY5 were compared. In addition, the DNA-binding properties of STF1 and HY5 were extensively studied using random binding site selection and electrophoretic mobility shift assay. Overexpression of STF1 in the hy5 mutant of Arabidopsis restored wild-type photomorphogenic and root development phenotypes of short hypocotyl, accumulation of chlorophyll, and root gravitropism with partial restoration of anthocyanin accumulation. This supports that STF1 is a homolog of HY5 with a role in light and hormone signaling. The DNA-binding properties of STF1 and HY5 are shown to be similar to each other in recognizing many ACGT-containing elements with a consensus sequence motif of 5'-(G/A)(G/A) TGACGT(C/G/A)(A/T/G)-3'. The motif represents a characteristically strong preference for flanking sequence to TGACGT and a larger sequence than the sequences recognized by the G-box binding factor and TGA protein families. The finding of C-box, hybrid C/G-, and C/A-boxes as high-affinity binding sites over the G-box and parameters associated with HY5 recognition define the criteria of HY5/STF1 protein-DNA interaction in the promoter regions. This study helps to predict the precise in vivo binding sites of the HY5 protein from the vast number of putative HY5 genomic binding sites analyzed by chromatin immunoprecipitation on chip.</description><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Root development</subject><subject>Site selection</subject><subject>Soybeans</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNjF1LwzAUhoMoWD_-w8H7YNIuEi-76FZhroMVZJQyKklmhiSapGB_iX_XUMRrr87Ded_3OUEZZUWOczbjpygjJDHh_P4cXYRwJITQgs4y9P2wLvHcWGnsAbZxkCM8SWWj0UYFEHjuvqC3Eqrx1RsJ4nY5vZxPWE747FI5QB-gMoc3XGptrIkj_ElNTCadFttmQSfZql4vodptalE3uxWDjXdRGRtoK7r2pWvrsrtCZ7p_D-r6916im8VjIyr84d3noELcH93gbYr2OeWM31HGin-VfgBOkFQv</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>Song, Young Hun</creator><creator>Yoo, Cheol Min</creator><creator>Hong, An Pio</creator><creator>Kim, Seong Hee</creator><creator>Jeong, Hee Jeong</creator><creator>Shin, Su Young</creator><creator>Kim, Hye Jin</creator><creator>Yun, Dae-Jin</creator><creator>Lim, Chae Oh</creator><creator>Bahk, Jeong Dong</creator><creator>Lee, Sang Yeol</creator><creator>Nagao, Ron T</creator><creator>Key, Joe L</creator><creator>Hong, Jong Chan</creator><general>American Society of Plant Biologists</general><scope>3V.</scope><scope>4T-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>20080401</creationdate><title>DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins1[C][W][OA]</title><author>Song, Young Hun ; Yoo, Cheol Min ; Hong, An Pio ; Kim, Seong Hee ; Jeong, Hee Jeong ; Shin, Su Young ; Kim, Hye Jin ; Yun, Dae-Jin ; Lim, Chae Oh ; Bahk, Jeong Dong ; Lee, Sang Yeol ; Nagao, Ron T ; Key, Joe L ; Hong, Jong Chan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_2185861553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Root development</topic><topic>Site selection</topic><topic>Soybeans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Young Hun</creatorcontrib><creatorcontrib>Yoo, Cheol Min</creatorcontrib><creatorcontrib>Hong, An Pio</creatorcontrib><creatorcontrib>Kim, Seong Hee</creatorcontrib><creatorcontrib>Jeong, Hee Jeong</creatorcontrib><creatorcontrib>Shin, Su Young</creatorcontrib><creatorcontrib>Kim, Hye Jin</creatorcontrib><creatorcontrib>Yun, Dae-Jin</creatorcontrib><creatorcontrib>Lim, Chae Oh</creatorcontrib><creatorcontrib>Bahk, Jeong Dong</creatorcontrib><creatorcontrib>Lee, Sang Yeol</creatorcontrib><creatorcontrib>Nagao, Ron T</creatorcontrib><creatorcontrib>Key, Joe L</creatorcontrib><creatorcontrib>Hong, Jong Chan</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Young Hun</au><au>Yoo, Cheol Min</au><au>Hong, An Pio</au><au>Kim, Seong Hee</au><au>Jeong, Hee Jeong</au><au>Shin, Su Young</au><au>Kim, Hye Jin</au><au>Yun, Dae-Jin</au><au>Lim, Chae Oh</au><au>Bahk, Jeong Dong</au><au>Lee, Sang Yeol</au><au>Nagao, Ron T</au><au>Key, Joe L</au><au>Hong, Jong Chan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins1[C][W][OA]</atitle><jtitle>Plant physiology (Bethesda)</jtitle><date>2008-04-01</date><risdate>2008</risdate><volume>146</volume><issue>4</issue><spage>1862</spage><pages>1862-</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>LONG HYPOCOTYL5 (HY5) is a bZIP (basic leucine zipper) transcription factor that activates photomorphogenesis and root development in Arabidopsis (Arabidopsis thaliana). Previously, STF1 (soybean [Glycine max] TGACG-motif binding factor 1), a homologous legume protein with a RING-finger motif and a bZIP domain, was reported in soybean. To investigate the role of STF1, the phenotypes of transgenic Arabidopsis plants overexpressing STF1 and HY5 were compared. In addition, the DNA-binding properties of STF1 and HY5 were extensively studied using random binding site selection and electrophoretic mobility shift assay. Overexpression of STF1 in the hy5 mutant of Arabidopsis restored wild-type photomorphogenic and root development phenotypes of short hypocotyl, accumulation of chlorophyll, and root gravitropism with partial restoration of anthocyanin accumulation. This supports that STF1 is a homolog of HY5 with a role in light and hormone signaling. The DNA-binding properties of STF1 and HY5 are shown to be similar to each other in recognizing many ACGT-containing elements with a consensus sequence motif of 5'-(G/A)(G/A) TGACGT(C/G/A)(A/T/G)-3'. The motif represents a characteristically strong preference for flanking sequence to TGACGT and a larger sequence than the sequences recognized by the G-box binding factor and TGA protein families. The finding of C-box, hybrid C/G-, and C/A-boxes as high-affinity binding sites over the G-box and parameters associated with HY5 recognition define the criteria of HY5/STF1 protein-DNA interaction in the promoter regions. This study helps to predict the precise in vivo binding sites of the HY5 protein from the vast number of putative HY5 genomic binding sites analyzed by chromatin immunoprecipitation on chip.</abstract><cop>Rockville</cop><pub>American Society of Plant Biologists</pub></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0032-0889 |
ispartof | Plant physiology (Bethesda), 2008-04, Vol.146 (4), p.1862 |
issn | 0032-0889 1532-2548 |
language | eng |
recordid | cdi_proquest_journals_218586155 |
source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
subjects | Deoxyribonucleic acid DNA Root development Site selection Soybeans |
title | DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins1[C][W][OA] |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-15T16%3A21%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA-Binding%20Study%20Identifies%20C-Box%20and%20Hybrid%20C/G-Box%20or%20C/A-Box%20Motifs%20as%20High-Affinity%20Binding%20Sites%20for%20STF1%20and%20LONG%20HYPOCOTYL5%20Proteins1%5BC%5D%5BW%5D%5BOA%5D&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Song,%20Young%20Hun&rft.date=2008-04-01&rft.volume=146&rft.issue=4&rft.spage=1862&rft.pages=1862-&rft.issn=0032-0889&rft.eissn=1532-2548&rft_id=info:doi/&rft_dat=%3Cproquest%3E1484840411%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=218586155&rft_id=info:pmid/&rfr_iscdi=true |