Evaluation of the growth of human fibroblasts on fibroin scaffolds from Bombyx mori L

The fibroin of Bombyx mori L., has been used for its physical / chemical characteristics as a biomaterial with applications in the healing of multiple tissues. In the context of regenerative medicine, characterizing at a physical and biological level new supports prepared from silk fibroin and evaluating their capacity for the proliferation of human fibroblasts, offers a very attractive opportunity to find new biomaterials with favorable applications in the healing of wounds. 17% regenerated fibroin was used for the manufacture of matrices. The matrices constructed were characterized in aspects such as: stability under growing conditions, ultra-structure, porosity, contact angle and mechanical properties. Finally, the ability of the scaffold to support the growth of human fibroblasts was evaluated. The average thickness of the fibroin matrices was 30.1 μm, with a stability greater than 4 weeks under culture conditions, porosity of 51% and a liquid retention capacity of 95%, a contact angle of 44.5 ° and a modulus of elasticity of ± 200 MPa. It was identified that the scaffolds allow cell multiplication, showing low cytotoxicity indexes (