Determination of staphylococcus aureus mycoprotein by using ELISA based on oscillating chemical kinetic detection

Oscillating chemical detection has the advantages of high sensitivity and fast and simple operation, but it is also very vulnerable to many additional species and has low selectivity, poor qualitative analysis and poor detection of macromolecules. This article establishes a Belousov–Zhabotinskii oscillating chemical detection system with good stability and repeatability, which involves ([CuL](ClO4)2) as a catalyst and C3H6O and C6H12O6 as a mixed substrate, as well as an enzyme-linked immunosorbent assay (ELISA) detection system, with disodium phenyl phosphate dihydrate (DPPD) as the key substrate and alkaline phosphatase (ALP) as a catalyst. This article introduces the oscillating chemical detection system into the enzyme-linked immune detection system and establishes the detection method, which combines the advantages of chemical oscillation with those of ELISA, including good selectivity and high specificity, to test the Staphylococcus aureus (S. aureus) protein. There is a good linear proportionality between the concentration of the S. aureus protein and the oscillating amplitude change ΔA, and the detection sensitivity of the method is much higher than that of normal spectrophotometry. The sensitivity of biotin-streptomycin ELISA with a detection limit (3σ) of 1.56 × 10−11 g/mL is better than that of double-antibody sandwich ELISA with a detection limit (3σ) of 1.06 × 10−9 g/mL. Therefore, this method can be applied to the detection of the S. aureus protein antigen. [Display omitted]