Identification of Native Flavin Adducts from Fusarium oxysporum Using Accurate Mass Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Accurate mass matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry is used to determine the identity of a naturally occurring substituted flavin adenine dinucleotide (FAD) of nitroalkane oxidase isolated from Fusarium oxysporum. Accurate mass measurements (1−2 p...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analytical chemistry (Washington) 1997-07, Vol.69 (14), p.2862-2865
Hauptverfasser: Edmondson, Ricky D, Gadda, Giovanni, Fitzpatrick, Paul F, Russell, David H
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Accurate mass matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry is used to determine the identity of a naturally occurring substituted flavin adenine dinucleotide (FAD) of nitroalkane oxidase isolated from Fusarium oxysporum. Accurate mass measurements (1−2 ppm) combined with an elemental composition search identifies the unknown FAD substituent as nitrobutane. The use of internal and external calibration in accurate mass MALDI-TOF is discussed.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac9700989