Identification of Native Flavin Adducts from Fusarium oxysporum Using Accurate Mass Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Accurate mass matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry is used to determine the identity of a naturally occurring substituted flavin adenine dinucleotide (FAD) of nitroalkane oxidase isolated from Fusarium oxysporum. Accurate mass measurements (1−2 p...
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Veröffentlicht in: | Analytical chemistry (Washington) 1997-07, Vol.69 (14), p.2862-2865 |
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Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Accurate mass matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry is used to determine the identity of a naturally occurring substituted flavin adenine dinucleotide (FAD) of nitroalkane oxidase isolated from Fusarium oxysporum. Accurate mass measurements (1−2 ppm) combined with an elemental composition search identifies the unknown FAD substituent as nitrobutane. The use of internal and external calibration in accurate mass MALDI-TOF is discussed. |
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ISSN: | 0003-2700 1520-6882 |
DOI: | 10.1021/ac9700989 |