Enhanced stability of urease immobilized onto phospholipid covalently bound to silica, tungsten, and fluoropolymer surfaces

Exceptionally high stability of urease covalently immobilized on silica, tungsten and poly-(tetrafluoroethylene) (Teflon) supports has been observed by using a novel immobilization protocol that links the enzyme via its surface-exposed carboxylic groups to phospholipid-coated surfaces. It was found that urease bound to lipid-coated silica or Teflon could be boiled in aqueous solution for one hour with minimal loss of activity.