Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection

A gel capillary-array electrophoresis system with real-time fluorescence detection that can be used to read nucleic acid bases at a speed of 200 per hour is described. Multiple sheath flows surround the 20 capillaries with buffer solution to prevent DNA band diffusion in the gel-free irradiated regi...

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Veröffentlicht in:	Analytical chemistry (Washington) 1994-04, Vol.66 (7), p.1021-1026
Hauptverfasser:	Takahashi, Satoshi, Murakami, Katsuhiko, Anazawa, Takashi, Kambara, Hideki
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Chemistry Deoxyribonucleic acid DNA Dna, deoxyribonucleoproteins Fundamental and applied biological sciences. Psychology Nucleic acids
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Zusammenfassung:	A gel capillary-array electrophoresis system with real-time fluorescence detection that can be used to read nucleic acid bases at a speed of 200 per hour is described. Multiple sheath flows surround the 20 capillaries with buffer solution to prevent DNA band diffusion in the gel-free irradiated region.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac00079a016