A Novel Approach to Septal Perforation Repair: Septal Cartilage Cells Induce Chondrogenesis of hASCs In Vitro
The aim of this study was to investigate the effect of medium harvested from septal cartilage cells on chondrogenic differentiation of adipose stem cells (hASCs) and to compare/contrast its properties to those of a commonly used standard medium formulation in terms of induction and maintenance of ch...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2019-08, Vol.188 (4), p.942-951 |
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| creator | Şafak, Ayşe Sezim Avşar Abdik, Ezgi Abdik, Hüseyin Taşlı, Pakize Neslihan Şahin, Fikrettin |
| description | The aim of this study was to investigate the effect of medium harvested from septal cartilage cells on chondrogenic differentiation of adipose stem cells (hASCs) and to compare/contrast its properties to those of a commonly used standard medium formulation in terms of induction and maintenance of chondrogenic hASCs. Differentiation was carried out under three different conditions: septal cartilage medium-SCM, chondrogenic differentiation medium-CM, and 50:50 mixture of CM/SCM. Mesenchymal stem cells (MSCs) markers were determined by flow cytometry. The cytotoxic and apoptotic effects were determined by MTS and Annexin V assay, respectively. The differentiation status of the cells was confirmed by Alcian blue staining, and quantitative real-time flow cytometry showed that hASCs were positive for MSCs, negative for hematopoietic stem cells and endothelial cell surface markers. According to MTS analysis, the first condition was not toxic at any concentration tested. Annexin V assay revealed that the application of different concentrations of SCM did not result in any cell death. The Alcian blue and gene expression analyses showed that the cells in the SCM group underwent the highest cartilage cell formation. The observed increase in chondrogenesis may offer better treatment options for the cartilage defects seen in nasal septum perforation. |
| doi_str_mv | 10.1007/s12010-019-02964-9 |
| format | Article |
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Differentiation was carried out under three different conditions: septal cartilage medium-SCM, chondrogenic differentiation medium-CM, and 50:50 mixture of CM/SCM. Mesenchymal stem cells (MSCs) markers were determined by flow cytometry. The cytotoxic and apoptotic effects were determined by MTS and Annexin V assay, respectively. The differentiation status of the cells was confirmed by Alcian blue staining, and quantitative real-time flow cytometry showed that hASCs were positive for MSCs, negative for hematopoietic stem cells and endothelial cell surface markers. According to MTS analysis, the first condition was not toxic at any concentration tested. Annexin V assay revealed that the application of different concentrations of SCM did not result in any cell death. The Alcian blue and gene expression analyses showed that the cells in the SCM group underwent the highest cartilage cell formation. The observed increase in chondrogenesis may offer better treatment options for the cartilage defects seen in nasal septum perforation.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-019-02964-9</identifier><identifier>PMID: 30740625</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Annexin V ; Apoptosis ; Biochemistry ; Biomarkers ; Biotechnology ; Cartilage ; Cell death ; Cell surface ; Chemistry ; Chemistry and Materials Science ; Chondrogenesis ; Cytotoxicity ; Differentiation ; Endothelial cells ; Flow cytometry ; Gene expression ; Hematopoietic stem cells ; Mesenchyme ; Perforation ; Septum ; Stem cells ; Surface markers ; Toxicity testing</subject><ispartof>Applied biochemistry and biotechnology, 2019-08, Vol.188 (4), p.942-951</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2019</rights><rights>Applied Biochemistry and Biotechnology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-f71fe6c4d5a1fb9885d11719553bf23e9926a6b78a354683327553c15168d9283</citedby><cites>FETCH-LOGICAL-c375t-f71fe6c4d5a1fb9885d11719553bf23e9926a6b78a354683327553c15168d9283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-019-02964-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-019-02964-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30740625$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Şafak, Ayşe Sezim</creatorcontrib><creatorcontrib>Avşar Abdik, Ezgi</creatorcontrib><creatorcontrib>Abdik, Hüseyin</creatorcontrib><creatorcontrib>Taşlı, Pakize Neslihan</creatorcontrib><creatorcontrib>Şahin, Fikrettin</creatorcontrib><title>A Novel Approach to Septal Perforation Repair: Septal Cartilage Cells Induce Chondrogenesis of hASCs In Vitro</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>The aim of this study was to investigate the effect of medium harvested from septal cartilage cells on chondrogenic differentiation of adipose stem cells (hASCs) and to compare/contrast its properties to those of a commonly used standard medium formulation in terms of induction and maintenance of chondrogenic hASCs. Differentiation was carried out under three different conditions: septal cartilage medium-SCM, chondrogenic differentiation medium-CM, and 50:50 mixture of CM/SCM. Mesenchymal stem cells (MSCs) markers were determined by flow cytometry. The cytotoxic and apoptotic effects were determined by MTS and Annexin V assay, respectively. The differentiation status of the cells was confirmed by Alcian blue staining, and quantitative real-time flow cytometry showed that hASCs were positive for MSCs, negative for hematopoietic stem cells and endothelial cell surface markers. According to MTS analysis, the first condition was not toxic at any concentration tested. Annexin V assay revealed that the application of different concentrations of SCM did not result in any cell death. The Alcian blue and gene expression analyses showed that the cells in the SCM group underwent the highest cartilage cell formation. The observed increase in chondrogenesis may offer better treatment options for the cartilage defects seen in nasal septum perforation.</description><subject>Annexin V</subject><subject>Apoptosis</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biotechnology</subject><subject>Cartilage</subject><subject>Cell death</subject><subject>Cell surface</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chondrogenesis</subject><subject>Cytotoxicity</subject><subject>Differentiation</subject><subject>Endothelial cells</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Hematopoietic stem cells</subject><subject>Mesenchyme</subject><subject>Perforation</subject><subject>Septum</subject><subject>Stem cells</subject><subject>Surface markers</subject><subject>Toxicity testing</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kEtPwzAQhC0EoqXwBzggS5wDXjuObW5VxKNSBYgCV8tNnDZVGgc7QeLfkz6AG6fVaGZntR9C50CugBBxHYASIBEBFRGqkjhSB2gInG8lHKIhoYJFlEo1QCchrAgBKrk4RgNGREwSyodoPcaP7tNWeNw03plsiVuHZ7ZpTYWfrS-cN23pavxiG1P6mx8rNb4tK7OwOLVVFfCkzrusF0tX594tbG1DGbAr8HI8Szc2fi9b707RUWGqYM_2c4Te7m5f04do-nQ_ScfTKGOCt1EhoLBJFufcQDFXUvIcQIDinM0LyqxSNDHJXEjDeJxIxqjorQw4JDJXVLIRutz19j99dDa0euU6X_cnNQUhSQxMqT5Fd6nMuxC8LXTjy7XxXxqI3hDWO8K6J6y3hPVm6WJf3c3XNv9d-UHaB9guEHqrXlj_d_uf2m_Ei4Rs</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Şafak, Ayşe Sezim</creator><creator>Avşar Abdik, Ezgi</creator><creator>Abdik, Hüseyin</creator><creator>Taşlı, Pakize Neslihan</creator><creator>Şahin, Fikrettin</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope></search><sort><creationdate>20190801</creationdate><title>A Novel Approach to Septal Perforation Repair: Septal Cartilage Cells Induce Chondrogenesis of hASCs In Vitro</title><author>Şafak, Ayşe Sezim ; Avşar Abdik, Ezgi ; Abdik, Hüseyin ; Taşlı, Pakize Neslihan ; Şahin, Fikrettin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-f71fe6c4d5a1fb9885d11719553bf23e9926a6b78a354683327553c15168d9283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Annexin V</topic><topic>Apoptosis</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Biotechnology</topic><topic>Cartilage</topic><topic>Cell death</topic><topic>Cell surface</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chondrogenesis</topic><topic>Cytotoxicity</topic><topic>Differentiation</topic><topic>Endothelial cells</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Hematopoietic stem cells</topic><topic>Mesenchyme</topic><topic>Perforation</topic><topic>Septum</topic><topic>Stem cells</topic><topic>Surface markers</topic><topic>Toxicity testing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Şafak, Ayşe Sezim</creatorcontrib><creatorcontrib>Avşar Abdik, Ezgi</creatorcontrib><creatorcontrib>Abdik, Hüseyin</creatorcontrib><creatorcontrib>Taşlı, Pakize Neslihan</creatorcontrib><creatorcontrib>Şahin, Fikrettin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Şafak, Ayşe Sezim</au><au>Avşar Abdik, Ezgi</au><au>Abdik, Hüseyin</au><au>Taşlı, Pakize Neslihan</au><au>Şahin, Fikrettin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Novel Approach to Septal Perforation Repair: Septal Cartilage Cells Induce Chondrogenesis of hASCs In Vitro</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2019-08-01</date><risdate>2019</risdate><volume>188</volume><issue>4</issue><spage>942</spage><epage>951</epage><pages>942-951</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>The aim of this study was to investigate the effect of medium harvested from septal cartilage cells on chondrogenic differentiation of adipose stem cells (hASCs) and to compare/contrast its properties to those of a commonly used standard medium formulation in terms of induction and maintenance of chondrogenic hASCs. Differentiation was carried out under three different conditions: septal cartilage medium-SCM, chondrogenic differentiation medium-CM, and 50:50 mixture of CM/SCM. Mesenchymal stem cells (MSCs) markers were determined by flow cytometry. The cytotoxic and apoptotic effects were determined by MTS and Annexin V assay, respectively. The differentiation status of the cells was confirmed by Alcian blue staining, and quantitative real-time flow cytometry showed that hASCs were positive for MSCs, negative for hematopoietic stem cells and endothelial cell surface markers. According to MTS analysis, the first condition was not toxic at any concentration tested. Annexin V assay revealed that the application of different concentrations of SCM did not result in any cell death. The Alcian blue and gene expression analyses showed that the cells in the SCM group underwent the highest cartilage cell formation. The observed increase in chondrogenesis may offer better treatment options for the cartilage defects seen in nasal septum perforation.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30740625</pmid><doi>10.1007/s12010-019-02964-9</doi><tpages>10</tpages></addata></record> |
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| ispartof | Applied biochemistry and biotechnology, 2019-08, Vol.188 (4), p.942-951 |
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| subjects | Annexin V Apoptosis Biochemistry Biomarkers Biotechnology Cartilage Cell death Cell surface Chemistry Chemistry and Materials Science Chondrogenesis Cytotoxicity Differentiation Endothelial cells Flow cytometry Gene expression Hematopoietic stem cells Mesenchyme Perforation Septum Stem cells Surface markers Toxicity testing |
| title | A Novel Approach to Septal Perforation Repair: Septal Cartilage Cells Induce Chondrogenesis of hASCs In Vitro |
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