The activity of Golgi transport vesicles depends on the presence of the N-ethylmaleimide-sensitive factor (NSF) and a soluble NSF attachment protein ((alpha)SNAP) during vesicle formation

The activity of Golgi transport vesicles depends on the presence of the N-ethylmaleimide-sensitive factor (NSF) and a soluble NSF attachment protein ((alpha)SNAP) during vesicle formation

An assay designed to measure the formation of functional transport vesicles is constructed by modifying a cell-free assay for protein transport between compartments of the Golgi. Soluble N-ethylmaleimide-sensitive factor attachment protein and N-ethylmaleimide-sensitive factor become incorporated in...

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Veröffentlicht in:The Journal of cell biology 1992-09, Vol.118 (6), p.1321
Hauptverfasser: Wattenberg, Binks W, Raub, Tom J, Hiebsch, Ron R, Weidman, Peggy J
Format: Artikel
Sprache:eng
Schlagworte:
Cell division
Membranes
Proteins
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Zusammenfassung:An assay designed to measure the formation of functional transport vesicles is constructed by modifying a cell-free assay for protein transport between compartments of the Golgi. Soluble N-ethylmaleimide-sensitive factor attachment protein and N-ethylmaleimide-sensitive factor become incorporated into the vesicle membranes during vesicle formation.
ISSN:0021-9525
1540-8140