SINGLE COTTON SEED DNA EXTRACTION WITHOUT THE USE OF ENZYMES AND LIQUID NITROGEN

DNA extraction is a routine procedure in most plant laboratories. Plant DNA isolation requires the use of several hazardous chemicals. The use of Proteinase K and RNase A contributes major isolation cost. Also the use of liquid nitrogen does not only increase the cost but also opens new ways of intr...

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Veröffentlicht in:	Fresenius environmental bulletin 2018-01, Vol.27 (10), p.6722
Hauptverfasser:	Aydin, Adnan, Ince, Ayse Gul, Gocer, Emine Uygur, Karaca, Mehmet
Format:	Artikel
Sprache:	eng
Schlagworte:	Contamination Cost effectiveness Cotton Deoxyribonucleic acid DNA Endopeptidase K Health risks Liquid nitrogen Molecular biology Next-generation sequencing Nitrogen Organic chemistry Polymerase chain reaction Proteinase
Online-Zugang:	Volltext
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