SINGLE COTTON SEED DNA EXTRACTION WITHOUT THE USE OF ENZYMES AND LIQUID NITROGEN

DNA extraction is a routine procedure in most plant laboratories. Plant DNA isolation requires the use of several hazardous chemicals. The use of Proteinase K and RNase A contributes major isolation cost. Also the use of liquid nitrogen does not only increase the cost but also opens new ways of introduction of contamination and injury risks. In the present study a CTAB-based cotton DNA extraction and purification method suitable for single cotton seed is reported. It is an inexpensive, rapid and simple procedure, especially when hundreds of cotton seed samples need to be analyzed. The main advantages of the method are high yield, high quality, and cost effectiveness. This method could satisfy demand when high amount plant genomic DNA required from large numbers of cotton plant samples. The quality (integrity, originality, and purity) and quantity (amount) of the extracted DNA are suitable for most molecular biology research including restriction enzyme digestion studies, polymerase chain reaction, next generation sequencing, and next generation marker technologies.