DIFFERENTIATED EFFECTS OF GLUCOSAMINYLMURAMILDIPEPTIDE ON THE NONTRANSFORMED AND EXPERIMENTALLY TRANSFORMED PHENOTYPE OF CD62L+CD63+CD66d+ NEUTROPHILIC GRANULOCYTES IN CONVENTIONALLY HEALTHY PEOPLE

Modern studies have shown a high plasticity and phenotypic diversity of neutrophilic granulocytes(NG) provided by different receptors, which are diagnostic markers for the functional capacity of the cellin the course of their activities. We investigated NG from peripheral blood, obtained from healthy people ofboth sexes aged from 26 to 66 years. Evaluation of the neutrophil membrane receptor expression was carriedout by flow cytometry. The relative amount of neutrophilic granulocytes expressing membrane CD62L,CD63, CD66d receptors and the intensity of their expression were determined according to their fluorescenceintensities. The surface NG membrane receptors, i.e., CD62L, CD63, CD66d were studied upon the in vitroexperimental influence of the following bacterial peptides: N-formyl-methionyl-leucyl-phenylalanine (FMLP,model 1); glucosaminylmuramyldipeptide (GMDP, model 2), and simultaneous incubation of NG blood withfMLP and GMDP (model 3). The in vitro treatment with fMLP in the in vitro model was used to transformthe NG phenotype of conventionally healthy subjects, expressing CD62, CD63, CD66d molecules. Thetreatment caused a significantly decrease in both CD62L and the CD62L expression in relative amounts ofneutrophilic granulocytes with a parallel increase of CD63 expression density. The effect of GMDP on the NGphenotype of conditionally healthy subjects did not change the amount of CD62L+NG and CD63+NG, anddid not affect CD62L and CD63 expression density on the surface of NG. However, the amount of CD66d+NGwas significantly increased with the unchanged expression of CD66d molecules. GMDP introduced togetherwith the bacterial fMLP peptide was shown to neutralize some features of the NG phenotype transformationcaused by fMLP, i.e., the amount of CD62L+ NG was restored by 22 % and the CD62L expression densityincreased significantly. At the same time, GMDP did not correct the negative effect of fMLP upon the numberof CD63+NG and CD66d+NG, and on the CD63 and CD66d expression. Simultaneous addition of fMLP and GMDP did significantly increase the amount of CD66d+NG and expression density of CD63 molecules on the CD63+NG membrane as compared to intact NG of conditionally healthy subjects. The obtained data are important in order to justify some new immunotherapeutic strategies aimed at correction of the negatively transformed NG phenotype, which accompanies some infectious and inflammatory diseases of bacterial etiology with atypical clinical course.