Effect of lipase from different source on high fat content wastewater of dairy industry

Wastewaters of dairy industry usually present high fat contents. The present study was designed to investigate the effect of different sources of lipase such as lipase contains raw milk, crude lipase from groundnuts extract, fat degrading microorganism from up flow anaerobic sludge blanket reactor (UASBR) culture in nutrient broth and lipase producing microorganism from UASBR culture in a selective media inoculated in holding tank dairy wastewater. Pretreatment of 250 ml dairy wastewater with 10 ml lipase contain sample was optimized under anaerobic condition for 72 hrs at 37ºC. Analytical parameters pH, fat content (FC), chemical oxygen demand (COD) and total solids (TS) were analysed in each treatment process. The results showed that pH of the samples were maintained, the UASBR culture inoculated in selective media degraded the maximum amount of fat; similarly maximum amount of COD and TS were reduced in the selective media treated sample as compared to others treatment process. This study illustrated that application of an enzymatic pretreatment process to hydrolyze and dissolve fats may improve the biological degradation of high fat content in wastewaters. Moreover, pretreatment of wastewater from different lipase sources are new and promising application for lipases. Thus, it is apparent that use of this enzymatic biological treatment can serve an alternative for treatment of Soybean casein digest Soybean casein digest Soybean casein digest Soybean casein digest fat containing wastewater and to provide pollution free environment.