Evaluation of Analgesic And Anti-inflammatory Activity of Herbal Formulation Used for Mastitis in Animals

Nature has been the source of medicinal agents for more than thousands of years, interestingly phytotherapy dominates in age old traditional system and also practice of alternative medicine across different cultures as Indian and Chinese system of medicine and Unani literature respectively. More than 70% of world's population is rely on herbal medicines for most part of their primary health care system. Investigations have been carried out from time to time to develop types of herbal formulations either singly or in combinations (Polyherbal formulations) to enhance overall therapeutic potential of the formulation. The present study was carried out to ascertain analgesic and anti-inflammatory activities of methanolic extracts of herbal combination containing equal proportion of Diploclisia glaucescens leaf and rhizomes of Curcuma longa in Wistar albino rats. The solvent yield of these extract was 16.75% and colour with dark brown to reddish brown with solid to semisolid consistency. The collected extracts were subjected to phytochemical analysis before animal experimentation, revealed the presence of all major phytoconstituents including alkaloids, flavonoids, saponins, glycosides, tannins and terpene compounds. The analgesic activity was assessed by Eddy's hot plate method and Formalin induced rat paw edema model and anti-inflammatory activity by Carrageenan induced paw edema model respectively. Significant analgesic and anti-inflammatory activity was noticed in the herbal formulation treated groups of both 200 and 400 mg/kg body weight. These test drug activity was sustained and comparable to the standard drug, Ibuprofen and Indomethacin. Thus the study can be concluded that the test drug possess significantly higher analgesic and anti-inflammatory activity possibly due to the presence of manifold secondary phytoconstituents.