PRODUCTION OF INTERLEUKIN 1BETA AND INTERFERON GAMMA BY BOVINE MAMMARY GLAND LEUKOCYTES STIMULATED BY LIPOPOLYSACCHARIDE

PRODUCTION OF INTERLEUKIN 1BETA AND INTERFERON GAMMA BY BOVINE MAMMARY GLAND LEUKOCYTES STIMULATED BY LIPOPOLYSACCHARIDE

The aim of this trial was to investigate the production of the pro-inflammatory cytokines interleukin 1beta (IL-1β) and interferon gamma (IFN-γ) produced by bovine mammary gland leukocytes following lipopolysaccharide (LPS) challenge (5 µg LPS of Escherichia coli in 20 ml of PBS) or PBS (20 ml) as a...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of microbiology, biotechnology and food sciences biotechnology and food sciences, 2018-12, Vol.8 (3), p.882-884
Hauptverfasser: Kabourkova, Eliska, Mika, Matej, Pavlik, Ales, Slama, Petr
Format: Artikel
Sprache:eng
Schlagworte:
Cattle
Cytokines
E coli
Enzyme-linked immunosorbent assay
IL-1β
Inflammation
Interferon
Interleukins
Leukocytes
Lipopolysaccharides
Mammary gland
Mammary glands
Stimulation
γ-Interferon
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The aim of this trial was to investigate the production of the pro-inflammatory cytokines interleukin 1beta (IL-1β) and interferon gamma (IFN-γ) produced by bovine mammary gland leukocytes following lipopolysaccharide (LPS) challenge (5 µg LPS of Escherichia coli in 20 ml of PBS) or PBS (20 ml) as a control. The leukocytes were obtained by mammary gland lavage 1, 2, 3, and 7 days following the stimulation. The concentration levels of these cytokines were analyzed by sandwich ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. Higher average number of leukocytes (P
ISSN:1338-5178
1338-5178
DOI:10.15414/jmbfs.2018-19.8.3.882-884