The effectivity of pandanus leaf extract for the treatment of sangkuriang catfish juvenile Clarias gariepinus infected by Aeromonas hydrophila

The aim of this study was to determine the effective concentration of pandanus Pandanus amaryllifolius leaf extract for the treatment of sangkuriang catfish juvenile infected by Aeromonas hydrophila. The method used in this study was an experimental method with completely randomized design consisted of five treatments and three replications. The treatments were A (control), B (400 mg/L), C (600 mg/L), D (800 mg/L), and E (1000 mg/L) trough immersion for 24 hours through immersion for 24 hours. The fish has infected with A. hydrophila bacteria as much as 20 mL of NaCl/L in 10 L of water medium with a density of 108 CFU/mL through immersion. The observed parameters were clinical symptoms, recovery process, survival rate, and water quality. The results showed that pandanus leaf extract at a concentration of 800 mg/L for 24 hours was effective for treating sangkuriang catfish juvenile that infected by A. hydrophila with a survival rate of 86.67%. Based on the regression analysis, it discovered that the optimum concentration of pandanus leaf extract through immersion for 24 hours was 774.39 mg/L and the predicted survival was 92.16%.